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Corn stover (1\u2009kg) was subjected to AHP pretreatment, hydrolyzed enzymatically, and the resulting sugars fermented to ethanol. The AHP pretreatment was performed at 0.125\u2009g H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\/g biomass, 22\u00b0C, and atmospheric pressure for 48\u2009h with periodic pH readjustment. The enzymatic hydrolysis was performed in the same reactor following pH neutralization of the biomass slurry and without washing. After 48\u2009h, glucose and xylose yields were 75% and 71% of the theoretical maximum. Sterility was maintained during pretreatment and enzymatic hydrolysis without the use of antibiotics. During fermentation using a glucose\u2010 and xylose\u2010utilizing strain of <jats:italic>Saccharomyces cerevisiae<\/jats:italic>, all of the Glc and 67% of the Xyl were consumed in 120\u2009h. The final ethanol titer was 13.7\u2009g\/L. Treatment of the enzymatic hydrolysate with activated carbon prior to fermentation had little effect on Glc fermentation but markedly improved utilization of Xyl, presumably due to the removal of soluble aromatic inhibitors. The results indicate that AHP is readily scalable and can be integrated with enzyme hydrolysis and fermentation. Compared to other leading pretreatments for lignocellulosic biomass, AHP has potential advantages with regard to capital costs, process simplicity, feedstock handling, and compatibility with enzymatic deconstruction and fermentation. Biotechnol. Bioeng. 2012; 109:922\u2013931. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.24385","type":"journal-article","created":{"date-parts":[[2011,11,28]],"date-time":"2011-11-28T08:52:01Z","timestamp":1322470321000},"page":"922-931","source":"Crossref","is-referenced-by-count":111,"title":["Scale\u2010up and integration of alkaline hydrogen peroxide pretreatment, enzymatic hydrolysis, and ethanolic fermentation"],"prefix":"10.1002","volume":"109","author":[{"given":"Goutami","family":"Banerjee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzana","family":"Car","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongjun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel 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