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GCR is a therapeutic protein used in the treatment of Gaucher disease, a life threatening condition in which patients lack sufficient functional levels of this enzyme. Gaucher disease is the most common inherited lysosomal storage disorder resulting in hepatomegaly, splenomegaly, and bone and lung pathology due to the accumulation of glucosylceramide in the lysosomes of macrophages (Beutler and Grabowski, ). The oligosaccharide remodeling of GCR, performed on HIC using three enzymes that remove sugars, increases macrophage uptake through the mannose receptor and thereby lowers its therapeutic dose versus unmodified GCR (Furbish et al., ; Van Patten et al., ). In this article we describe findings that the addition of HES lowered the amounts of three deglycosylating enzymes needed for remodeling GCR. HES also stabilized the activity of \u03b1\u2010glucosidase, \u03b1\u2010galactosidase, and GCR under conditions in which these three enzymes rapidly lose activity in the absence of this polymer. Circular dichroism (CD) and second derivative UV spectroscopy revealed that the secondary and tertiary structure of \u03b1\u2010glucosidase was unchanged while for GCR there was a slight compaction of the secondary structure but no apparent affect on the tertiary structure. The thermal stability of both GCR and \u03b1\u2010glucosidase were enhanced by HES as both molecules showed an increased transition midpoint (T<jats:sub>m<\/jats:sub>). Biotechnol. Bioeng. 2012; 109:1217\u20131227. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.24406","type":"journal-article","created":{"date-parts":[[2011,12,13]],"date-time":"2011-12-13T08:01:22Z","timestamp":1323763282000},"page":"1217-1227","source":"Crossref","is-referenced-by-count":1,"title":["Remodeling the oligosaccharides on \u03b2\u2010glucocerebrosidase using hydrophobic interaction chromatography and applications of hydroxyl ethyl starch for improving remodeling and enhancing protein stability"],"prefix":"10.1002","volume":"109","author":[{"given":"Frank","family":"Riske","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Hamilton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mike","family":"Hayes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,12,26]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(78)90102-2"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp993506g"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.1986.6.687"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(83)90201-1"},{"key":"e_1_2_5_6_1","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0021-9258(18)72124-8","article-title":"The influence of sugars on the formation of sulfhydryl groups in heat denaturation and heat coagulation of egg albumin","volume":"151","author":"Ball CD","year":"1943","journal-title":"J Biol Chem"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199105233242104"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.5.1913"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm0609487"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0268-960X(88)90009-4"},{"key":"e_1_2_5_11_1","first-page":"3635","volume-title":"The metabolic and molecular bases of inherited disease","author":"Beutler E","year":"2001"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560010907"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-66-4-887"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007920100236"},{"key":"e_1_2_5_15_1","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1016\/S0021-9258(19)51348-5","article-title":"The prevention by caprylate of urea and guanidine denaturation of serum albumin","volume":"158","author":"Boyer PD","year":"1945","journal-title":"J Biol Chem"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.9.2373"},{"key":"e_1_2_5_17_1","first-page":"241","volume-title":"Compatible solutes. 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