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Two complementary approaches were applied: predicting trends in the microbial community structure based on an electron balance analysis and experimentally assessing the community structure via pyrosequencing and quantitative polymerase chain reaction (qPCR). Fill\u2010and\u2010draw reactors inoculated with the DehaloR\u20412 consortium were operated at five TCE\u2010pulsing rates between 14 and 168\u2009\u00b5mol\/10\u2010day\u2010SRT, amended with TCE every 2 days to give peak concentrations between 0.047 and 0.56\u2009mM (6\u201374\u2009ppm) and supplied lactate and methanol as sources of e<jats:sup>\u2010<\/jats:sup> donor and carbon. The complementary approaches demonstrated the same trends: increasing abundance of <jats:italic>Dehalococcoides<\/jats:italic> and <jats:italic>Geobacter<\/jats:italic> and decreasing abundance of <jats:italic>Firmicutes<\/jats:italic> with increasing TCE pulsing rate, except for the highest pulsing rate. Based on qPCR, the abundance of <jats:italic>Geobacter<\/jats:italic> and <jats:italic>Dehalococcoides<\/jats:italic> decreased for the highest TCE pulsing rate, and pyrosequencing showed this same trend for the latter. This deviation suggested decoupling of <jats:italic>Dehalococcoides<\/jats:italic> growth from dechlorination. At pseudo steady\u2010state, methanogenesis was minimal for all TCE pulsing rates. Pyrosequencing and qPCR showed suppression of the homoacetogenic genera <jats:italic>Acetobacterium<\/jats:italic> at the two highest pulsing rates, and it was corroborated by a decreased production of acetate from lactate fermentation and increased propionate production. Suppression of <jats:italic>Acetobacterium<\/jats:italic>, which can provide growth factors to <jats:italic>Dehalococcoides<\/jats:italic>, may have contributed to the decoupling for the highest TCE\u2010pulsing rate. Biotechnol. 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