{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T22:38:36Z","timestamp":1788388716192,"version":"build-2803163510"},"reference-count":58,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,9,18]],"date-time":"2012-09-18T00:00:00Z","timestamp":1347926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It has recently been reported that 2\u2010methyl\u20102,4\u2010pentanediol (MPD) can modulate the protein\u2010binding properties of sodium dodecyl sulfate (SDS), turning it into a non\u2010denaturing detergent. Indeed both alpha (the lysozyme) and beta (the carbonic anhydrase II) soluble enzymes, as well as a beta membrane protein (PagP) have been successfully refolded into their native form by using this amphiphatic alcohol. In order to support the universal character of our MPD\u2010based technique, we have extended its transferability to the Omp2a trimeric membrane porin. The far\u2010UV circular dichroism signature of Omp2a refolded with our original procedure is identical to that obtained by classical techniques, clearly indicating a proper refolding. Moreover, we show that the optimal SDS\/MPD ratio for refolding Omp2a is similar to what has been observed for other types of proteins. While the protocol allows refolding at higher protein concentration (up to 4\u2009mg\/mL) and ionic strength (up to 1\u2009M NaCl) than other refolding methods, it is also more efficient at basic pH values and medium temperature (20\u201340\u00b0C). Finally, the key role of the cosolvent was highlighted by a thorough study of the efficiency of MPD analogues, and a high variability was observed, as they can be able or unable to induce refolding at low or high salt concentrations. Biotechnol. Bioeng. 2013; 110: 417\u2013423. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.24722","type":"journal-article","created":{"date-parts":[[2012,9,4]],"date-time":"2012-09-04T12:12:21Z","timestamp":1346760741000},"page":"417-423","source":"Crossref","is-referenced-by-count":18,"title":["Towards a universal method for protein refolding: The trimeric beta barrel membrane Omp2a as a test case"],"prefix":"10.1002","volume":"110","author":[{"given":"Guillaume","family":"Roussel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric A.","family":"Perp\u00e8te","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Matagne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Tinti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Michaux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0927-7757(99)00089-8"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(02)00711-3"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1656(98)00197-7"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.21318"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2144\/000113739"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9797(88)90332-3"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2736(02)00714-9"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(00)00165-X"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04395"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M802754200"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9797(82)90271-5"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/j100402a033"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2011.08.006"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.2144\/000112517"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/j150606a021"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2010.06.014"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2007.06.001"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.44.1.16-21.1984"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi100339x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/jcis.1994.1154"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(89)90602-7"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00225a008"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602816103"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.07.072"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2006.12.004"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-8067-5"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.105.077867"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi961478b"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-003-3170-0"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4157(00)00010-1"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0491898"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00664a020"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.11.026"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6807-8-29"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0169567"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.101238"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(02)73982-9"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2000.10506564"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.16.4839-4847.2001"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813167106"},{"key":"e_1_2_6_42_1","doi-asserted-by":"crossref","first-page":"7753","DOI":"10.1016\/S0021-9258(18)68563-1","article-title":"Targeting of porin to the outer membrane of Escherichia coli. Rate of trimer assembly and identification of a dimer intermediate","volume":"263","author":"Reid J","year":"1988","journal-title":"J Biol Chem"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00855a038"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2012.04.003"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cis.2006.05.018"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2004.04.011"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20041102"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1021\/j100094a022"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.47.28199"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi951216u"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2004.06.011"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2012.01.013"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/la981234n"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-2508-7_3"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20051257"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10930-009-9165-4"},{"key":"e_1_2_6_57_1","first-page":"531","article-title":"Protein identification and analysis tools in the ExPASy server","volume":"112","author":"Wilkins MR","year":"1999","journal-title":"Methods Mol Biol"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00615-07"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2011.09.015"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.24722","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.24722","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T14:15:46Z","timestamp":1759932946000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.24722"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9,18]]},"references-count":58,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1002\/bit.24722"],"URL":"https:\/\/doi.org\/10.1002\/bit.24722","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9,18]]}}}