{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:37:52Z","timestamp":1791405472361,"version":"4.3.3"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,2,4]],"date-time":"2013-02-04T00:00:00Z","timestamp":1359936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Microalgae, with their high lipid content, are a promising feedstock for renewable fuels. Traditionally, human and environmentally toxic solvents have been used to extract these lipids, diminishing the sustainability of this process. Herein, pulsed electric field technology was utilized as a process intensification strategy to enhance lipid extraction from\n                    <jats:italic>Ankistrodesmus falcatus<\/jats:italic>\n                    wet biomass using the green solvent, ethyl acetate. The extraction efficiency for ethyl acetate without PEF was lower (83\u201388%) than chloroform. In addition, the ethyl acetate exhibited a 2\u2010h induction period, while the chloroform showed no time dependence. Utilizing PEF technology resulted in 90% of the cells being lysed and a significant enhancement in the rate of lipid recovery using ethyl acetate. The increase in lipid recovery was due to the presence of the electric field and not due to temperature effects. The PEF technology uses less energy than other PEF systems reported in the literature. Biotechnol. Bioeng. 2013; 110: 1605\u20131615. \u00a9 2013 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/bit.24829","type":"journal-article","created":{"date-parts":[[2013,1,7]],"date-time":"2013-01-07T09:17:11Z","timestamp":1357550231000},"page":"1605-1615","source":"Crossref","is-referenced-by-count":221,"title":["Pulsed electric field (PEF) as an intensification pretreatment for greener solvent lipid extraction from microalgae"],"prefix":"10.1002","volume":"110","author":[{"given":"Mauricio D. Antezana","family":"Zbinden","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Belinda S.M.","family":"Sturm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan D.","family":"Nord","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"Carey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Moore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Shinogle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan M.","family":"Stagg\u2010Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,2,4]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"BarclayW JohansenJ ChelfP NagleN RoesslerP LemkeP.1986. Microalgae culture collection 1986\u20131987. Solar Energy Research Inst. Golden CO (USA). Report nr SERI\/SP\u2014232\u20133079. 153 p."},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/es102052y"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0010(20000901)80:11<1668::AID-JSFA692>3.0.CO;2-7"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1139\/y59-099"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf802579x"},{"issue":"1216","key":"e_1_2_6_7_1","first-page":"194","article-title":"Beet juice extraction by pressing and pulsed electric fields","volume":"102","author":"Bouzrara H","year":"2000","journal-title":"Int Sugar J"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/b617536h"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apenergy.2011.04.018"},{"key":"e_1_2_6_10_1","first-page":"1","article-title":"Extraction and conversion pathways for microalgae to biodiesel: A review focused on energy consumption","author":"de Boer K","year":"2012","journal-title":"J Appl Phycol"},{"key":"e_1_2_6_11_1","unstructured":"EckelberryND GreenMP FraserSA United States Patent Application Publication.2011. Systems apparatuses and methods for extracting non\u2010polar lipids from an aqueous algae slurry and lipids produced therefrom. USA patent US 2011\/0095225 A1."},{"key":"e_1_2_6_12_1","first-page":"2806","volume-title":"Comprehensive biotechnology: The principles, applications and regulations of biotechnology, in industry, agriculture and medicine","author":"Emery A","year":"1985"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.2172\/6782425"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-6946(02)00038-9"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-8714-9_3"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/j150543a028"},{"key":"e_1_2_6_17_1","unstructured":"KempkesMA RothI GaudreauMPJ United States Patent Application Publication.2011. Pulsed electric field (PEF) method for continuous enhanced extraction of oil and lipids from small aquatic plants. USA patent US 2011\/0107655 A1."},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/ie990669i"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ie801542g"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/es900705j"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008811716448"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2009.03.058"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02542261"},{"key":"e_1_2_6_24_1","first-page":"1","article-title":"Principles of membrane electroporation and transport of macromolecules","volume":"37","author":"Neumann E","year":"2000","journal-title":"Methods Mol Med"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/ie9016557"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1109\/TPS.2008.2002440"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11367-010-0194-1"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0732713"},{"key":"e_1_2_6_29_1","first-page":"141","article-title":"Extraction and purification of lipids: II. Why is chloroform\u2013methanol such a good lipid solvent","volume":"5","author":"Schmid P","year":"1973","journal-title":"Physiol Chem Phys"},{"key":"e_1_2_6_30_1","first-page":"98","article-title":"Extraction and purification of lipids. I. Solubility of lipids in biologically important solvents","volume":"3","author":"Schmid P","year":"1971","journal-title":"Physiol Chem Phys"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ifset.2007.06.012"},{"key":"e_1_2_6_32_1","unstructured":"SheehanJ DunahayT BenemannJ RoesslerP.1998. A look back at the US Department of Energy's Aquatic Species Program\u2010Biodiesel from Algae. National Renweable Energy Laboratory. NREL\/TP\u2010580\u201024190 Golden CO."},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/es103339x"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2010.08.007"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.2172\/6757339"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2005.05.006"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1080\/87559120600865164"},{"key":"e_1_2_6_38_1","first-page":"115","article-title":"Lipid production by Dunaliella salina in batch culture: Effects of nitrogen limitation and light intensity","volume":"7","author":"Weldy CS","year":"2007","journal-title":"US DOE J Undergrad Res"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(94)80805-7"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.24829","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.24829","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T18:15:08Z","timestamp":1759947308000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.24829"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2,4]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1002\/bit.24829"],"URL":"https:\/\/doi.org\/10.1002\/bit.24829","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,2,4]]}}}