{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T16:30:08Z","timestamp":1784133008655,"version":"3.55.0"},"reference-count":20,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,3,31]],"date-time":"2013-03-31T00:00:00Z","timestamp":1364688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Escherichia coli<\/jats:italic> cells co\u2010expressing genes coding for <jats:italic>Candida tenuis<\/jats:italic> xylose reductase and <jats:italic>Candida boidinii<\/jats:italic> formate dehydrogenase were used for the bioreduction of <jats:italic>o<\/jats:italic>\u2010chloroacetophenone with in situ coenzyme recycling. The product, (<jats:italic>S<\/jats:italic>)\u20101\u2010(2\u2010chlorophenyl)ethanol, is a key chiral intermediate in the synthesis of polo\u2010like kinase 1 inhibitors, a new class of chemotherapeutic drugs. Production of the alcohol in multi\u2010gram scale requires intensification and scale\u2010up of the biocatalyst production, biotransformation, and downstream processing. Cell cultivation in a 6.9\u2010L bioreactor led to a more than tenfold increase in cell concentration compared to shaken flask cultivation. The resultant cells were used in conversions of 300\u2009mM substrate to (<jats:italic>S<\/jats:italic>)\u20101\u2010(2\u2010chlorophenyl)ethanol (e.e. &gt;99.9%) in high yield (96%). Results obtained in a reaction volume of 500\u2009mL were identical to biotransformations carried out in 1\u2009mL (analytical) and 15\u2009mL (preparative) scale. Optimization of product isolation based on hexane extraction yielded 86% isolated product. Biotransformation and extraction were accomplished in a stirred tank reactor equipped with pH and temperature control. The developed process lowered production costs by 80% and enabled (<jats:italic>S<\/jats:italic>)\u20101\u2010(2\u2010chlorophenyl)ethanol production within previously defined economic boundaries. A simple and efficient way to synthesize (<jats:italic>S<\/jats:italic>)\u20101\u2010(2\u2010chlorophenyl)ethanol in an isolated amount of 20\u2009g product per reaction batch was demonstrated. Biotechnol. Bioeng. 2013; 110: 2311\u20132315. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/bit.24896","type":"journal-article","created":{"date-parts":[[2013,3,8]],"date-time":"2013-03-08T10:24:10Z","timestamp":1362738250000},"page":"2311-2315","source":"Crossref","is-referenced-by-count":20,"title":["Scale\u2010up and intensification of (<i>S<\/i>)\u20101\u2010(2\u2010chlorophenyl)ethanol bioproduction: Economic evaluation of whole cell\u2010catalyzed reduction of <i>o<\/i>\u2010Chloroacetophenone"],"prefix":"10.1002","volume":"110","author":[{"given":"Thomas","family":"Eixelsberger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Woodley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd","family":"Nidetzky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina","family":"Kratzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,3,31]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00449-002-0281-1"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-010-0130-x"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200503394"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2004.08.011"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(19971005)56:1<83::AID-BIT9>3.0.CO;2-R"},{"key":"e_1_2_4_7_1","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/S1389-1723(04)70218-2","article-title":"Scale\u2010up methodologies for Escherichia coli and yeast fermentation processes","volume":"7","author":"Junker BH","year":"2004","journal-title":"J Biosci Bioeng"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.23002"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00758518"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-11-7"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.16.1547"},{"key":"e_1_2_4_12_1","unstructured":"NaglG.1999.Optimierung eines fermentationsprozesses zur erzeugung des rekombinanten enzyms mannitol\u2010dehydrogenase inEscherichia coli. 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