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Amphiphilic polymer conetworks (APCNs) have been shown to greatly activate entrapped enzymes in organic solvents. Since these nanostructured materials are not porous, the bioactivity of the conetworks is strongly limited by diffusion of substrate and product. The present manuscript describes two different APCNs as nanostructured microparticles, which showed greatly increased activities of entrapped enzymes compared to those of the already activating membranes and larger particles. We demonstrated this on the example of APCN particles based on PHEA\u2010<jats:italic>l<\/jats:italic>\u2010PDMS loaded with <jats:italic>\u03b1<\/jats:italic>\u2010Chymotrypsin, which resulted in an up to 28,000\u2010fold higher activity of the enzyme compared to the enzyme powder. Furthermore, lipase from <jats:italic>Rhizomucor miehei<\/jats:italic> entrapped in particles based on PHEA\u2010<jats:italic>l<\/jats:italic>\u2010PEtOx was tested in <jats:italic>n<\/jats:italic>\u2010heptane, chloroform, and substrate. Specific activities in smaller particles were 10\u2010 to 100\u2010fold higher in comparison to the native enzyme. The carrier activity of PHEA\u2010<jats:italic>l<\/jats:italic>\u2010PEtOx microparticles was tenfold higher with some 25\u201350\u2010fold lower enzyme content compared to a commercial product. Biotechnol. Bioeng. 2013; 110:2333\u20132342. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bit.24906","type":"journal-article","created":{"date-parts":[[2013,3,26]],"date-time":"2013-03-26T11:23:35Z","timestamp":1364297015000},"page":"2333-2342","source":"Crossref","is-referenced-by-count":51,"title":["Investigations on diffusion limitations of biocatalyzed reactions in amphiphilic polymer conetworks in organic solvents"],"prefix":"10.1002","volume":"110","author":[{"given":"Ina","family":"Schoenfeld","sequence":"first","affiliation":[{"name":"Department of Bio\u2010 and Chemical Engineering TU Dortmund Emil\u2010Figge\u2010Str. 66 D\u201044227 Dortmund Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephan","family":"Dech","sequence":"additional","affiliation":[{"name":"Department of Bio\u2010 and Chemical Engineering TU Dortmund Emil\u2010Figge\u2010Str. 66 D\u201044227 Dortmund 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