{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T19:25:51Z","timestamp":1788895551952,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,5,12]],"date-time":"2015-05-12T00:00:00Z","timestamp":1431388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"the National Science Foundation of China","award":["31270100, 31070027"],"award-info":[{"award-number":["31270100, 31070027"]}]},{"name":"the Open Funding Project of the State Key Laboratory of Bio-organic and Natural Products Chemistry"},{"name":"the Open Funding Project of the State Key Laboratory of Bioreactor Engineering"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Polyoxin and nikkomycin are naturally occurring peptidyl nucleoside antibiotics with potent antifungal bioactivity. Both exhibit similar structural features, having a nucleoside skeleton and one or two peptidyl moieties. Combining the refactoring of the polyoxin producer\n                      <jats:italic>Streptomyces aureochromogenes<\/jats:italic>\n                      with import of the hydroxypyridylhomothreonine pathway of nikkomycin allows the targeted production of three designer nucleoside antibiotics designated as nikkoxin E, F, and G. These structures were determined by NMR and\/or high resolution mass spectrometry. Remarkably, the introduction of an extra copy of the\n                      <jats:italic>nikS<\/jats:italic>\n                      gene encoding an ATP\u2010dependent ligase significantly enhanced the production of the designer antibiotics. Moreover, all three nikkoxins displayed improved bioactivity against several pathogenic fungi as compared with the naturally\u2010occurring antibiotics. These data provide a feasible model for high efficiency generation of nucleoside antibiotics related to polyoxins and nikkomycins in a polyoxin cell factory\n                      <jats:italic>via<\/jats:italic>\n                      synthetic biology strategy. Biotechnol. Bioeng. 2015;112: 1865\u20131871. \u00a9 2015 Wiley Periodicals, Inc.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/bit.25594","type":"journal-article","created":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T02:52:23Z","timestamp":1427856743000},"page":"1865-1871","source":"Crossref","is-referenced-by-count":12,"title":["Metabolic engineering of an industrial polyoxin producer for the targeted overproduction of designer nucleoside antibiotics"],"prefix":"10.1002","volume":"112","author":[{"given":"Jianzhao","family":"Qi","sequence":"first","affiliation":[{"name":"Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences Wuhan University Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences Wuhan University Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Wan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences Wuhan University Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You\u2010sheng","family":"Cai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences Wuhan University Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinghu","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences Wuhan University Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunying","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences Wuhan University Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences Wuhan University Wuhan 430071 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Feng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences Wuhan University Wuhan 430071 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Science and Technology Shanghai 200237 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixin","family":"Deng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences Wuhan University Wuhan 430071 China"},{"name":"State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai 200030 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