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The resting cell enzyme system acted as a stable respiratory unit up to 150 hr after resuspension in water. Semicontinuous addition of substrate to the same cell suspension reduced overall conversion lime by 67%.<\/jats:p><jats:p>Aeration and agitation were important factors affecting conversion rates. The hydroxylating system had a critical oxygen concentration above 90% saturation of air in water. Hydroxylase activity was inhibited by cyanide an d totally inhibited by the respiratory inhibitor antimycin A at concentrations much less that that concentration required to block normal respiration.<\/jats:p>","DOI":"10.1002\/bit.260140512","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T20:43:00Z","timestamp":1104352980000},"page":"831-841","source":"Crossref","is-referenced-by-count":8,"title":["Pilot plant conversion of steroid using resting cell suspension as biocatalyst"],"prefix":"10.1002","volume":"14","author":[{"given":"W. 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