{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:02:38Z","timestamp":1760644958472,"version":"build-2065373602"},"reference-count":13,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2004,2,18]],"date-time":"2004-02-18T00:00:00Z","timestamp":1077062400000},"content-version":"vor","delay-in-days":9605,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[1977,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The application of the enzyme thermistor in the analysis of cyanide in standard solutions as well as in blast furnace waste water is described. The heat signal is generated in the conversion of cyanide, catalyzed by the immobilized enzymes rhodanese (E.C. 2.8.1.1) and injectase (E.C. 4.4.19). Using the combination of cyanide\u2010metabolizing enzymes and the enzyme thermistor unit, assays down to 20\u03bc<jats:italic>M<\/jats:italic> cyanide can be carried out. Linear relationships were obtained at 20\u2013600\u03bc<jats:italic>M<\/jats:italic> cyanide for injectase and 20\u20131000\u03bc<jats:italic>M<\/jats:italic> for rhodanese. The stability at 27\u00b0C of the heat response was initially decreased, but soon stabilized at about 80% of the initial value and remained so for at least 200 hr. The technique was easily adapted to continuous analysis, applicable to environmental control (e.g., a \u201ccyanide guard\u201d) with a response time at present within 2\u20133 min after a sudden change in cyanide concentration has appeared.<\/jats:p>","DOI":"10.1002\/bit.260191104","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T14:56:28Z","timestamp":1104332188000},"page":"1643-1651","source":"Crossref","is-referenced-by-count":24,"title":["Application of cyanide\u2010metabolizing enzymes to environmental control; enzyme thermistor assay of cyanide using immobilized rhodanese and injectase"],"prefix":"10.1002","volume":"19","author":[{"given":"Bo","family":"Mattiasson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Mosbach","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"Svenson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,18]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0039-9140(64)80073-4"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/00139307109435426"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/es60114a011"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1351\/pac197125040727"},{"key":"e_1_2_1_6_2","first-page":"377","volume":"2","author":"Clark L. C.","year":"1972","journal-title":"Biotechnol. Bioeng. Symp."},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(73)90333-4"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2744(75)90027-3"},{"key":"e_1_2_1_9_2","volume":"9","author":"Mattiasson B.","year":"1976","journal-title":"Anal. Lett."},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(73)90131-1"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/00032717608059098"},{"key":"e_1_2_1_12_2","unstructured":"A.SvensonandB.Andersson Anal. 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