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No activity of surface active agent in whole broth was observed when glucose was used in the MMSM instead of hexadecane. The biomass concentration obtained with glucose was about 40% of that obtained with hexadecane. Glucose (4%) in the medium contaning hexadecane caused a 27 and 21% decrease of biomass and surfactant concentrations, respectively. In the process of surfactant production, glucose can be used as a carbon source for growth, and hexadecane added later can serve for production of the surface active agent. The optimum temperature for production of surfactant is 27\u00b0C.<\/jats:p>","DOI":"10.1002\/bit.260240114","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T22:39:00Z","timestamp":1104359940000},"page":"165-175","source":"Crossref","is-referenced-by-count":49,"title":["Production of surfactant by <i>Arthrobacter paraffineus<\/i> ATCC 19558"],"prefix":"10.1002","volume":"24","author":[{"given":"Z.","family":"Duvnjak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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