{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:10:44Z","timestamp":1791252644652,"version":"4.1.0"},"reference-count":10,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2004,2,18]],"date-time":"2004-02-18T00:00:00Z","timestamp":1077062400000},"content-version":"vor","delay-in-days":7779,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[1982,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Death of <jats:italic>Saccharomyces cerevisiae<\/jats:italic> induced by sulfur dioxide (K<jats:sub>2<\/jats:sub>S<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> was used as the SO<jats:sub>2<\/jats:sub> source) followed saturation kinetics. The enthalpy of activation of death was not affected by concentration over the range tested (5\u2013150) mg\/L of (K<jats:sub>2<\/jats:sub>S<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> at pH 3.4) and averaged 3.6 \u00d7 10<jats:sup>4<\/jats:sup> cal\/mol as compared with 8.5 \u00d7 10<jats:sup>4<\/jats:sup> cal\/mol for \u0394<jats:italic>H<\/jats:italic><jats:sup>\u2021<\/jats:sup> of thermal death. The entropy of activation of death was hyperbolic function of the sulfur dioxide concentration, extrapolated at zero concentration to \u0394<jats:italic>S<\/jats:italic><jats:sub>0<\/jats:sub><jats:sup>\u2021<\/jats:sup> = 36.8 cal mol<jats:sup>\u22121<\/jats:sup> K<jats:sup>\u22121<\/jats:sup> and tended to \u0394\u0394<jats:italic>S<\/jats:italic><jats:sub>max<\/jats:sub><jats:sup>\u2021<\/jats:sup> = 13.2 cal mol<jats:sup>\u22121<\/jats:sup> K<jats:sup>\u22121<\/jats:sup> at saturating concentration, yielding a dis0sociation constant of 5.8 \u00d7 10<jats:sup>\u22121<\/jats:sup> <jats:italic>M<\/jats:italic> sulfur dioxide. As was predicted from these results, In <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub> (the specific rate of death induced by sulfur dioxide) was hyperbolic function of concentration under isothermic conditions and extrapolated toa finite value at zero concentration. The Arrhenuis plots and the \u0394<jats:italic>S<\/jats:italic><jats:sup>\u00b1<\/jats:sup> plot versus concentration revealed the occurrence of substrate inhabitation of the death effect at high concentrations (above 60 mg\/L K<jats:sub>2<\/jats:sub>S<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> at pH 3.4). A model is presented involving two types of receptor sites for sulfur dioxide on the cell surface, on directly connected with the death process, the other modulating its entropy of activation.<\/jats:p>","DOI":"10.1002\/bit.260241112","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T22:14:01Z","timestamp":1104358441000},"page":"2477-2486","source":"Crossref","is-referenced-by-count":15,"title":["Kinetics and activation energetics of death in <i>Saccharomyces cerevisiae<\/i> induced by sulfur dioxide"],"prefix":"10.1002","volume":"24","author":[{"given":"J.","family":"Anacleto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"van Uden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,2,18]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00425059"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00403203"},{"key":"e_1_2_1_4_2","unstructured":"B.Macris \u201cTransport and toxicity of sulfur dioxide in the yeastSacharomyces cerevisiae \u201d Ph.D. thesis University of Michigan 1972."},{"key":"e_1_2_1_5_2","first-page":"248","volume-title":"Die Hefen","author":"Uhl A.","year":"1960"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00406676"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1039\/jr9600003711"},{"key":"e_1_2_1_8_2","first-page":"D 130","volume-title":"Handbook of chemistryu and physics","author":"Weast R. C.","year":"1974"},{"key":"e_1_2_1_9_2","volume-title":"The Kinetic Basis of Molecular Biology","author":"Johnson F. H.","year":"1954"},{"key":"e_1_2_1_10_2","volume-title":"Biometry","author":"Sokal R. R.","year":"1966"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00454855"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.260241112","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.260241112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:09:10Z","timestamp":1760054950000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.260241112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,11]]},"references-count":10,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1982,11]]}},"alternative-id":["10.1002\/bit.260241112"],"URL":"https:\/\/doi.org\/10.1002\/bit.260241112","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,11]]}}}