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The EG activity(measured as carboxymethyl cellulose hydrolysis) was separated by ion exchange chromatography into three distinct components which differ in their kinetic properties. Exposure of <jats:italic>Thm. curvata<\/jats:italic> to ultraviolet light, <jats:italic>N<\/jats:italic>\u2010nitrosoguanidine, or ethane methyl sulfonate produced mutants with enhanced EG production. Selection of colonies which cleared cellulose agar plant containing 2\u2010deoxtglucose of glycerol yielded mutants having 1.5 to 2.6 times the extracellular EG and saccharifying activity (measured by filter\u2010paper and cotton\u2010fiber hydrolysis). The secretion of extracellular protein was increased proportionally in mutant cultures.<\/jats:p>","DOI":"10.1002\/bit.260241113","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T22:14:01Z","timestamp":1104358441000},"page":"2487-2497","source":"Crossref","is-referenced-by-count":28,"title":["Enhanced cellulase production in mutants of <i>Thermomonospora curvata<\/i>"],"prefix":"10.1002","volume":"24","author":[{"given":"George","family":"Fennington","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davis","family":"Lupo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fred","family":"Stutzenberger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,18]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"103","volume":"8","author":"Cooney C. 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