{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:48:55Z","timestamp":1760060935627,"version":"build-2065373602"},"reference-count":10,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,2,18]],"date-time":"2004-02-18T00:00:00Z","timestamp":1077062400000},"content-version":"vor","delay-in-days":6207,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[1987,2,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Krill chitin modified with carbon disulfide was used as a support of enzymes. The dithiocarbamino groups created after reaction of CS<jats:sub>2<\/jats:sub> with chitinous NH<jats:sub>2<\/jats:sub> can participate in binding the enzymes without denaturation and loss in activity. The stability of these bonds depends on pH and decreases gradually at ionic strengths higher than 0.01. Chitin modified with CS<jats:sub>2<\/jats:sub> can be used as a support for enzymes having high activity at mild conditions of pH and ionic strength.<\/jats:p>","DOI":"10.1002\/bit.260290309","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T22:16:33Z","timestamp":1104358593000},"page":"352-354","source":"Crossref","is-referenced-by-count":7,"title":["Adsorption of enzymes on krill chitin modified with carbon disulfide"],"prefix":"10.1002","volume":"29","author":[{"given":"J\u00f3zef","family":"Synowiecki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Sikorska\u2010Siondalska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abo El\u2010Fath","family":"El\u2010Bedawey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,18]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260231005"},{"key":"e_1_2_1_3_2","first-page":"199","volume-title":"Chitin","author":"Muzzarelli R. A. A.","year":"1977"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260181011"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260180314"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408397909527276"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0308-8146(82)90025-5"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01480305"},{"key":"e_1_2_1_9_2","unstructured":"E.HayesandD. H.Davies inProceedings of the First International Conference on Chitin\/Chitosan Cambridge 1978 p.182."},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(59)90090-6"},{"key":"e_1_2_1_11_2","first-page":"110","volume-title":"Displacement of Water and its Control of Biochemical Reactions","author":"Levin S.","year":"1974"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.260290309","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.260290309","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:16:05Z","timestamp":1760037365000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.260290309"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,2,20]]},"references-count":10,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1987,2,20]]}},"alternative-id":["10.1002\/bit.260290309"],"URL":"https:\/\/doi.org\/10.1002\/bit.260290309","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"type":"print","value":"0006-3592"},{"type":"electronic","value":"1097-0290"}],"subject":[],"published":{"date-parts":[[1987,2,20]]}}}