{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T00:13:45Z","timestamp":1789776825760,"version":"4.0.1"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":4581,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[1991,8,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Methanotrophs have promising applications in bioremediation and in the production of fuel\u2010related chemicals due to their nonspecific enzyme, methane monooxygenase (MMO). The optimal conditions for cell growth and production of the soluble from of MMO (sMMO) were determined from batch cultivations of an obligatory methanotrophs, <jats:italic>Methylosinus trichosporium<\/jats:italic> OB3b, in shake flasks and a 5\u2010L bioreactor. It was confirmed that a copper deficiency is essential for the formation of the cytoplasmic sMNO. Optimum cell growth without added copper was observed at pH 6.0\u20137.0, temperature of 30\u201334\u00b0C, and phosphate concentration of 10\u201340 m<jats:italic>M<\/jats:italic>. In the bioreactor experiments, external CO<jats:sub>2<\/jats:sub> addition eliminated the long lag period observed in the absence of added CuSO<jats:sub>4<\/jats:sub>, i.e., prior to the exponential cell growth phase. When methane was continuously supplied, the profile of the cell growth showed two different phases depending on the availability of nitrate, an initial fast exponential growth phase (specific growth rate, \u03bc = 0.08 h<jats:sup>\u22121<\/jats:sup>) and a later slow growth phase (\u03bc = 0.008 h<jats:sup>\u22121<\/jats:sup>). The cell density at the transition from a fast to a slow growth rate was proportional to the initial medium nitrate concentration in the range 5\u201320 m<jats:italic>M<\/jats:italic> and cell yield was estimated to be 7.14 g dry cell wt\/g N. Whole\u2010cell sMNO activity remained essentially constant regardless of the growth rate unit cell growth stopped. With an initial medium iron concentration below 40 m<jats:italic>M<\/jats:italic>, an abrupt decrease in sMNO activity was observed. The lower sMNO activity could be restored by supplying additional iron to the bioreactor culture. Cell yield on iron was estimated to be 1.3 \u00d7 10<jats:sup>3<\/jats:sup> g dry cell wt\/g Fe.<\/jats:p>","DOI":"10.1002\/bit.260380412","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T19:01:36Z","timestamp":1104346896000},"page":"423-433","source":"Crossref","is-referenced-by-count":86,"title":["Batch cultivation of <i>Methylosinus trichosporium<\/i> OB3b. I: Production of soluble methane monooxygenase"],"prefix":"10.1002","volume":"38","author":[{"given":"Sunghoon","family":"Park","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie","family":"Hanna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert T.","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael W.","family":"Droege","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"The Biochemistry of Methylotrophs","author":"Anthony C.","year":"1982"},{"key":"e_1_2_1_3_2","unstructured":"Boutacoff D.1989.EPRI Journal Oct\/Nov:24."},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.10.4778-4783.1987"},{"key":"e_1_2_1_5_2","first-page":"3123","volume":"134","author":"Chen Y.\u2010P.","year":"1988","journal-title":"J. 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