{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T12:07:31Z","timestamp":1767960451892,"version":"3.49.0"},"reference-count":12,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":3666,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[1994,2,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Methanococcus jannaschii<\/jats:italic>, an extremely thermophilic methanogen isolated from a deep\u2010sea hydrothermal vent, was grown at 80\u00b0C in continuous culture on a mineral salts medium gassed with H<jats:sub>2<\/jats:sub> and CO<jats:sub>2<\/jats:sub> at three different flow rates. The maximum specific growth rate was 0.56 h<jats:sup>\u22121<\/jats:sup>, and the maximum specific methane productivity was 0.32 (mol g<jats:sup>\u22121<\/jats:sup> h<jats:sup>\u22121<\/jats:sup>). Uncoupling of growth and methane production was evidenced by an increase in teh non\u2010growth\u2010associated rate of methane formation, \u03b2, with increasing gaseous input. The specific hydrogenase activity exhibited growth\u2010assiciated behaviour at low growth rates, but showed no dependence on growth at higher growth rates. The growth dependence of hydrogenase activity is consistent with the pressure dependence of hydrogenase activity measured in previous experiments. In contrast, the specific protease activity was independent of the growth rate over the entire range of dilution rates studied. \u00a9 1994 John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/bit.260430309","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T23:51:26Z","timestamp":1104364286000},"page":"258-261","source":"Crossref","is-referenced-by-count":32,"title":["Continuous culture of <i>Methanococcus jannaschii<\/i>, an extremely thermophilic methanogen"],"prefix":"10.1002","volume":"43","author":[{"given":"Jia\u2010Huey","family":"Tsao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stacey M.","family":"Kaneshiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu\u2010San","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas S.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1128\/aem.32.6.781-791.1976","article-title":"New approach to teh cultivation of methanogenic bacteria:2\u2010Mercaptoethanesulfonic acid (HS\u2010CoM)\u2010dependent growth of Methanobacterium ruminantium in a pressurized atmosphere","volume":"32","author":"Balch W. 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