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Addition of either Ni and H<jats:sub>2<\/jats:sub> to a nitrate\u2010containing minimal salts growth medium or Ni and Mo to a nitrate\u2010lacking growth medium (induces a nitrogenase that generates intracellular H<jats:sub>2<\/jats:sub>) markedly enhanced both the hydrogenase and the accompanying washed\u2010cell H<jats:sub>2<\/jats:sub>\u2010driven MMO activities of shake\u2010flask cultured cells. For sMMO containing cells, H<jats:sub>2<\/jats:sub> provided in vitro reducing power for the oxidation of chlorinated solvents such as chloroform and trichloroethylene. Cell cultivations under N<jats:sub>2<\/jats:sub>\u2010fixing conditions in a 5\u2010L bioreactor, however, required an initial nitrate concentration of at least 1 to 2 m<jats:italic>M<\/jats:italic> to achieve high biomass yields (5 to 7 g of dry cell wt\/L) for cells producing H<jats:sub>2<\/jats:sub>\u2010driven sMMO or pMMO activity. Elevation of the initial medium nitrate concentration to 20 m<jats:italic>M<\/jats:italic> shortened the culture time for pMMO producing cells by 40%, yet still generated an equivalent growth yield. High nitrate also shortened the culture time for sMMO containing cells by \u223c25%, but it lowered their biomass yield by 26%. Upon storage for 5 weeks at room temperature, washed resting\u2010state cells retained 90% and 70% of their H<jats:sub>2<\/jats:sub>\u2010driven sMMO and pMMO activity, respectively. This makes their practical use quite feasible. \u00a9 1995 John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/bit.260450307","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T12:52:47Z","timestamp":1104324767000},"page":"229-238","source":"Crossref","is-referenced-by-count":31,"title":["Batch cultivation of <i>Methylosinus trichosporium<\/i> OB3B: IV. 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