{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:27:29Z","timestamp":1790792849670,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T00:00:00Z","timestamp":1077148800000},"content-version":"vor","delay-in-days":3301,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[1995,2,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hydrogen\u2010oxidizing bacterium, <jats:italic>Alcaligenes eutrophus<\/jats:italic> autotrophically produces biodegradable plastic material, poly(<jats:sc>D<\/jats:sc>\u20103\u2010hydroxybutyrate), P(3HB), from carbon dioxide, hydrogen, and oxygen. In autotrophic cultivation of the microorganism, it is essential to eliminate possible occurrence of gas explosions from the fermentation process. We developed a bench\u2010plant scale, recycled\u2010gas, closed\u2010circuit culture system equipped with several safety features to perform autotrophic cultivation of <jats:italic>A. eutrophus<\/jats:italic> by maintaining the oxygen concentration in the substrate gas phase below the lower limit for a gas explosion (6.9%). The culture vessel utilized a baskettype agitator, resulting in a <jats:italic>K<\/jats:italic><jats:sub>L<\/jats:sub> a value of 2970 h<jats:sup>\u22121<\/jats:sup>. Oxygen gas was also directly fed to the fermentor separately from the other gases. As a result, 91.3 g \u00b7 dm<jats:sup>\u22123<\/jats:sup> of the cells and 61.9 g \u00b7 dm<jats:sup>\u22123<\/jats:sup> of P(3HB) were obtained after 40 h of cultivation under this oxygen\u2010limited condition. The results compared favorably with those reported for mass production of P(3HB) by heterotrophic fermentation. \u00a9 1995 John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/bit.260450312","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T12:52:47Z","timestamp":1104324767000},"page":"268-275","source":"Crossref","is-referenced-by-count":167,"title":["Production of poly(<scp>D<\/scp>\u20103\u2010hydroxybutyrate) from CO<sub>2<\/sub>, H<sub>2<\/sub>, and O<sub>2<\/sub> by high cell density autotrophic cultivation of <i>Alcaligenes eutrophus<\/i>"],"prefix":"10.1002","volume":"45","author":[{"given":"Kenji","family":"Tanaka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayaaki","family":"Ishizaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihisa","family":"Kanamaru","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeharu","family":"Kawano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,2,19]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.54.4.450-472.1990"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/ie50414a005"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb1961.41.521"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1271\/nogeikagaku1924.60.935"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0922-338X(90)90041-T"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0922-338X(91)90277-N"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01029144"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260430908"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260240514"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1271\/bbb1961.42.439"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0922-338X(91)90164-C"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.3136\/nskkk1962.36.11_924"},{"key":"e_1_2_1_14_2","first-page":"4236","volume":"1","author":"Page W. 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