{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T09:45:01Z","timestamp":1780479901744,"version":"3.54.1"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,3,14]],"date-time":"2018-03-14T00:00:00Z","timestamp":1520985600000},"content-version":"am","delay-in-days":330,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2017,4,18]],"date-time":"2017-04-18T00:00:00Z","timestamp":1492473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000149","name":"Division of Engineering Education and Centers","doi-asserted-by":"publisher","award":["EEC\u2010\u00c2\u00ad0813570"],"award-info":[{"award-number":["EEC\u2010\u00c2\u00ad0813570"]}],"id":[{"id":"10.13039\/100000149","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech &amp;amp; Bioengineering"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:label\/><jats:p>Biorenewable chemicals such as short and medium chain fatty acids enable functional or direct substitution of petroleum\u2010derived building blocks, allowing reduction of anthropogenic greenhouse gases while meeting market needs of high\u2010demand products like aliphatic alcohols and alpha olefins. However, producing these fatty acids in microorganisms can be challenging due to toxicity issues. Octanoic acid (C8) can disrupt the integrity of the cell membrane in yeast, and exogenous supplementation of oleic acid has been shown to help alleviate this. We recently engineered the <jats:italic>Saccharomyces cerevisiae<\/jats:italic> enzyme acetyl\u2010CoA carboxylase by replacing serine residue 1157 with alanine to prevent deactivation by phosphorylation. Expression of Acc1<jats:sup>S1157A<\/jats:sup> in <jats:italic>S. cerevisiae<\/jats:italic> resulted in an increase in total fatty acid production, with the largest increase for oleic acid. In this study, we evaluated the effect of this modified lipid profile on C8 toxicity to the yeast. Expression of Acc1<jats:sup>S1157A<\/jats:sup> in <jats:italic>S. cerevisiae<\/jats:italic> BY4741 increased the percentage of oleic acid 3.1\u2010 and 1.6\u2010fold in the absence and presence of octanoic acid challenge, respectively. Following exposure to 0.9\u2009mM of C8 for 24\u2009h, the engineered yeast had a 10\u2010fold higher cell density relative to the baseline strain. Moreover, overexpressing Acc1<jats:sup>S1157A<\/jats:sup> allowed survival at C8 concentrations that were lethal for the baseline strain. This marked reduction of toxicity was shown to be due to higher membrane integrity as an 11\u2010fold decrease in leakage of intracellular magnesium was observed. Due to the increase in oleic acid, this approach has the potential to reduce toxicity of other valuable bioproducts such as shorter chain aliphatic acids and alcohols and other membrane stressors. In an initial screen, increased resistance to <jats:italic>n<\/jats:italic>\u2010butanol, 2\u2010propanol, and hexanoic acid was demonstrated with cell densities 3.2\u2010, 1.8\u2010, and 29\u2010fold higher than the baseline strain, respectively. Biotechnol. Bioeng. 2017;114: 1531\u20131538. \u00a9 2017 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bit.26288","type":"journal-article","created":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T17:23:16Z","timestamp":1489512196000},"page":"1531-1538","source":"Crossref","is-referenced-by-count":60,"title":["Engineering <i>Saccharomyces cerevisiae<\/i> fatty acid composition for increased tolerance to octanoic acid"],"prefix":"10.1002","volume":"114","author":[{"given":"Pamela B.","family":"Besada\u2010Lombana","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering and Materials Science University of California Irvine California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruben","family":"Fernandez\u2010Moya","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Materials Science University of California Irvine California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob","family":"Fenster","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Materials Science University of California Irvine California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3570-0037","authenticated-orcid":false,"given":"Nancy A.","family":"Da Silva","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Materials Science University of California Irvine California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-142-3-469"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.320111006"},{"key":"e_1_2_6_4_1","first-page":"8","article-title":"Differentiation between yeast species, and strains within a species, by cellular fatty acid analysis. Saccharomyces cerevisiae","volume":"10","author":"Augustyn OPH","year":"1989","journal-title":"South African J Enol Vitic"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(00)00159-9"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.3071"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(19980130)14:2<115::AID-YEA204>3.0.CO;2-2"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2015.03.025"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002030100269"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/15567249.2010.516310"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2008.00128.x"},{"key":"e_1_2_6_12_1","first-page":"42","article-title":"U. S. Biofuels Industry: A critical review of opportunities and challenges","volume":"2025","author":"Chen M","year":"2015","journal-title":"Bioprod Bussinessiness"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2014.07.430"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enpol.2013.10.079"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02319-16"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cherd.2015.10.035"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7827(90)90002-3"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7827(90)90002-3"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2014.04.025"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a121964"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00272"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2014.11.006"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01280-10"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.32"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-013-4773-5"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-015-1606-9"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601204"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1637"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2010.0072"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jim.7000090"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.26093"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.2050-0416.1964.tb01986.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2006.00230.x"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-10-2635"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(03)00226-7"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.59.2.304-322.1995"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1989.tb04220.x"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01360-06"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2016.06.005"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2015.12.017"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00732047"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005444322823"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-011-3410-4"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.3.1499-1503.2003"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1839"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-013-4698-z"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11709"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.26288","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.26288","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bit.26288","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/am-pdf\/10.1002\/bit.26288","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.26288","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T20:50:07Z","timestamp":1759956607000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.26288"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,18]]},"references-count":46,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1002\/bit.26288"],"URL":"https:\/\/doi.org\/10.1002\/bit.26288","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,18]]}}}