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BST\u20102 (tetherin) is a host restriction factor which inhibits budding\u2010out of various enveloped viruses from infected host cells. We developed BST\u20102\u2010deficient MDCK and Vero cell lines to increase influenza virus release in cell culture. BST\u20102 gene knock\u2010out resulted in increased release of viral particles into the culture medium, by at least 2\u2010fold and up to 50\u2010fold compared to release from wild\u2010type counterpart cells depending on cell line and virus type. The effect was not influenza virus\/MDCK\/Vero\u2010specific, but was also present in a broad range of host cells and virus families; we observed similar results in murine, human, canine, and monkey cell lines with viruses including MHV\u201068 (Herpesviridae), influenza A virus (Orthomyxoviridae), porcine epidemic diarrhea virus (Coronaviridae), and vaccinia virus (Poxviridae). 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