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This paper seeks to use an existing validated ultra scale\u2010down tool, which is designed to mimic the commercial scale process environment using only milliliters of material, to provide some initial insight into the performance of the harvest step for these processes. The performance of industrial scale centrifugation and subsequent downstream process units is significantly affected by shear. The properties of these cells, in particular their shear sensitivity, may be changed considerably by production of a viral product, but literature on this is limited to date. In addition, the scale\u2010down tool used here has not previously been applied to the clarification of virus production processes. The results indicate that virus infected cells do not actually show any increase in sensitivity to shear, and may indeed become less shear sensitive, in a similar manner to that previously observed in old or dead cell cultures. Clarification may be most significantly dependent on the virus release mechanism, with the budding influenza virus producing a much greater decrease in clarification than the lytic, non\u2010enveloped adenovirus. A good match was also demonstrated to the industrial scale performance in terms of clarification, protein release, and impurity profile.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bit.26546","type":"journal-article","created":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T02:59:29Z","timestamp":1515553169000},"page":"1226-1238","source":"Crossref","is-referenced-by-count":2,"title":["Ultra scale\u2010down approaches to study the centrifugal harvest for viral vaccine production"],"prefix":"10.1002","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2721-3941","authenticated-orcid":false,"given":"Beatrice J.","family":"Melinek","sequence":"first","affiliation":[{"name":"Department of Biochemical Engineering Bernard Katz building, University College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandrine","family":"Dessoy","sequence":"additional","affiliation":[{"name":"GSK Vaccines Rixensart Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernice","family":"Wright","sequence":"additional","affiliation":[{"name":"Department of Biochemical Engineering Bernard Katz building, University College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan G.","family":"Bracewell","sequence":"additional","affiliation":[{"name":"Department of Biochemical Engineering Bernard Katz building, University College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarit K.","family":"Mukhopadhyay","sequence":"additional","affiliation":[{"name":"Department of Biochemical Engineering Bernard Katz building, University College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/10_008"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.2217\/fvl.11.93"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp070305i"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0290(20000705)69:1<1::AID-BIT1>3.0.CO;2-4"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00449-003-0328-y"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-2509(01)00139-7"},{"key":"e_1_2_9_8_1","unstructured":"CELL_BIOLABS_Inc. 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