{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:17:20Z","timestamp":1791613040859,"version":"4.3.4"},"reference-count":99,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,8,11]],"date-time":"2018-08-11T00:00:00Z","timestamp":1533945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008868","name":"Science and Technology Department of Jiangsu Province","doi-asserted-by":"crossref","award":["BE2017342"],"award-info":[{"award-number":["BE2017342"]}],"id":[{"id":"10.13039\/501100008868","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"Chinese National Science Foundation","doi-asserted-by":"crossref","award":["31072136"],"award-info":[{"award-number":["31072136"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"Chinese National Science Foundation","doi-asserted-by":"crossref","award":["31270171"],"award-info":[{"award-number":["31270171"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"Chinese National Science Foundation","doi-asserted-by":"crossref","award":["30571374"],"award-info":[{"award-number":["30571374"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"Chinese National Science Foundation","doi-asserted-by":"crossref","award":["31672579"],"award-info":[{"award-number":["31672579"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"Chinese National Science Foundation","doi-asserted-by":"crossref","award":["30771603"],"award-info":[{"award-number":["30771603"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Priority Academic Program of Development Jiangsu High Education Institution"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Due to the inherent expression stability and low metabolic burden to the host cell, the expression of heterologous proteins in the bacterial chromosome in a precise and efficient manner is highly desirable for metabolic engineering and live bacterial applications. However, obtaining suitable chromosome expression levels is particularly challenging. In this minireview, we briefly present the technologies available for the integration of heterologous genes into <jats:italic>Escherichia coli<\/jats:italic> chromosomes and strategies to optimize the expression levels of heterologous proteins.<\/jats:p>","DOI":"10.1002\/bit.26790","type":"journal-article","created":{"date-parts":[[2018,7,7]],"date-time":"2018-07-07T10:38:54Z","timestamp":1530959934000},"page":"2467-2478","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Techniques for chromosomal integration and expression optimization in <i>Escherichia coli<\/i>"],"prefix":"10.1002","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5047-0364","authenticated-orcid":false,"given":"Bingming","family":"Ou","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Important Animal Infectious Diseases and Zoonoses Yangzhou China"},{"name":"Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety of Ministry of Education of China, Yangzhou University Yangzhou China"},{"name":"Diagnostic Medicine\/Pathobiology Kansas State University College of Veterinary Medicine Manhattan Kansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Garcia","sequence":"additional","affiliation":[{"name":"Diagnostic Medicine\/Pathobiology Kansas State University College of Veterinary Medicine Manhattan Kansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yejun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Genetics School of Basic Medical Sciences, Shenzhen University Health Science Center Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Diagnostic Medicine\/Pathobiology Kansas State University College of Veterinary Medicine Manhattan Kansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5491-0670","authenticated-orcid":false,"given":"Guoqiang","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yangzhou University Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Important Animal Infectious Diseases and Zoonoses Yangzhou China"},{"name":"Joint International Research Laboratory of Agriculture and Agri\u2010Product Safety of Ministry of Education of China, Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,11]]},"reference":[{"key":"e_1_2_6_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-011-3416-y"},{"key":"e_1_2_6_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.26530"},{"key":"e_1_2_6_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(88)90440-4"},{"key":"e_1_2_6_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13579"},{"key":"e_1_2_6_1_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.5b00187"},{"issue":"4","key":"e_1_2_6_1_7_1","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1128\/jvi.15.4.861-871.1975","article-title":"Transfection of Escherichia coli spheroplasts. V. Activity of recBC nuclease in rec\u2009+\u2009and rec minus spheroplasts measured with different forms of bacteriophage DNA","volume":"15","author":"Benzinger R.","year":"1975","journal-title":"Journal of Virology"},{"key":"e_1_2_6_1_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt520"},{"key":"e_1_2_6_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260370808"},{"key":"e_1_2_6_1_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks694"},{"key":"e_1_2_6_1_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053952"},{"key":"e_1_2_6_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro787"},{"key":"e_1_2_6_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.103"},{"key":"e_1_2_6_1_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku828"},{"key":"e_1_2_6_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201200085"},{"key":"e_1_2_6_1_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/bp9801143"},{"key":"e_1_2_6_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02428117"},{"key":"e_1_2_6_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2515"},{"key":"e_1_2_6_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21976"},{"key":"e_1_2_6_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2016.08.002"},{"key":"e_1_2_6_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.26056"},{"key":"e_1_2_6_1_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.8.2110"},{"key":"e_1_2_6_1_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.11.3240"},{"key":"e_1_2_6_1_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.36.061102.093104"},{"key":"e_1_2_6_1_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_2_6_1_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq810"},{"key":"e_1_2_6_1_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09886"},{"key":"e_1_2_6_1_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1536"},{"key":"e_1_2_6_1_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.6b00350"},{"key":"e_1_2_6_1_30_1","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20134955"},{"key":"e_1_2_6_1_31_1","volume-title":"Transduction: host DNA transfer by bacteriophages","author":"Fineran P.","year":"2009"},{"key":"e_1_2_6_1_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/b12440"},{"key":"e_1_2_6_1_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.090527097"},{"key":"e_1_2_6_1_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep09684"},{"key":"e_1_2_6_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2004.04.006"},{"key":"e_1_2_6_1_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.21.6384-6393.2001"},{"key":"e_1_2_6_1_37_1","first-page":"111","volume-title":"Antibiotic\u2010free plasmid selection and maintenance in bacteria. Recombinant protein production with prokaryotic and eukaryotic cells. A Comparative view on host physiology","author":"Hanak J. A.","year":"2001"},{"key":"e_1_2_6_1_38_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.37.110801.142807"},{"key":"e_1_2_6_1_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.11.6557-6567.1990"},{"key":"e_1_2_6_1_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/b93994"},{"key":"e_1_2_6_1_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt986"},{"key":"e_1_2_6_1_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2508"},{"key":"e_1_2_6_1_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.04023-14"},{"key":"e_1_2_6_1_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.synbio.2017.10.003"},{"key":"e_1_2_6_1_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2014.11.013"},{"key":"e_1_2_6_1_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.2185"},{"key":"e_1_2_6_1_47_1","doi-asserted-by":"publisher","DOI":"10.1006\/mben.2000.0161"},{"key":"e_1_2_6_1_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.12296"},{"key":"e_1_2_6_1_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0111451"},{"key":"e_1_2_6_1_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq934"},{"key":"e_1_2_6_1_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2012.06.009"},{"key":"e_1_2_6_1_52_1","doi-asserted-by":"publisher","DOI":"10.4161\/bbug.1.4.12386"},{"key":"e_1_2_6_1_53_1","doi-asserted-by":"publisher","DOI":"10.4161\/bbug.1.4.12386"},{"key":"e_1_2_6_1_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp1193"},{"key":"e_1_2_6_1_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2018.02.007"},{"key":"e_1_2_6_1_56_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-11-19"},{"key":"e_1_2_6_1_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2015.06.006"},{"key":"e_1_2_6_1_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2401"},{"key":"e_1_2_6_1_59_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00365-13"},{"key":"e_1_2_6_1_60_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.4.2140-2144.2005"},{"key":"e_1_2_6_1_61_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-8-63"},{"key":"e_1_2_6_1_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(00)00071-8"},{"key":"e_1_2_6_1_63_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0340203"},{"key":"e_1_2_6_1_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2404"},{"key":"e_1_2_6_1_65_1","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kvd049"},{"key":"e_1_2_6_1_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-016-7829-5"},{"key":"e_1_2_6_1_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(84)90059-3"},{"key":"e_1_2_6_1_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1226"},{"key":"e_1_2_6_1_69_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.12187"},{"key":"e_1_2_6_1_70_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2859-12-60"},{"key":"e_1_2_6_1_71_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1568"},{"key":"e_1_2_6_1_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(06)21015-2"},{"key":"e_1_2_6_1_73_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.6.2872-2875.1987"},{"key":"e_1_2_6_1_74_1","doi-asserted-by":"publisher","DOI":"10.1021\/sb400058n"},{"key":"e_1_2_6_1_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.227"},{"key":"e_1_2_6_1_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2015.24"},{"key":"e_1_2_6_1_77_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-143-6-2071"},{"key":"e_1_2_6_1_78_1","doi-asserted-by":"publisher","DOI":"10.1021\/sb400021j"},{"key":"e_1_2_6_1_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(86)90385-2"},{"key":"e_1_2_6_1_80_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb0116s78"},{"key":"e_1_2_6_1_81_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1555"},{"key":"e_1_2_6_1_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2012.03.013"},{"key":"e_1_2_6_1_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.06.035"},{"key":"e_1_2_6_1_84_1","doi-asserted-by":"publisher","DOI":"10.1385\/MB:32:1:043"},{"key":"e_1_2_6_1_85_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02178-16"},{"key":"e_1_2_6_1_86_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-010-2674-4"},{"key":"e_1_2_6_1_87_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2016.02.010"},{"key":"e_1_2_6_1_88_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2017.10.009"},{"key":"e_1_2_6_1_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2012.08.010"},{"key":"e_1_2_6_1_90_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.6b00187"},{"key":"e_1_2_6_1_91_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2425"},{"key":"e_1_2_6_1_92_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00313-14"},{"key":"e_1_2_6_1_93_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-015-1878-1"},{"key":"e_1_2_6_1_94_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-014-5959-1"},{"key":"e_1_2_6_1_95_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-015-6510-8"},{"key":"e_1_2_6_1_96_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-321-9_17"},{"key":"e_1_2_6_1_97_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.100127597"},{"key":"e_1_2_6_1_98_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2005.08.005"},{"key":"e_1_2_6_1_99_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11426-016-0194-x"},{"key":"e_1_2_6_1_100_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature26141"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.26790","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.26790","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bit.26790","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.26790","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T13:02:54Z","timestamp":1759928574000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.26790"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,11]]},"references-count":99,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2018,10]]}},"alternative-id":["10.1002\/bit.26790"],"URL":"https:\/\/doi.org\/10.1002\/bit.26790","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,11]]},"assertion":[{"value":"2018-04-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}