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In this study, we aim to develop a 2D (two\u2010dimensional) in vitro cardiac hypoxia model mimicking severe cardiac ischemia to specifically investigate the prosurvival paracrine effects of adipose tissue\u2010derived stromal vascular fraction (SVF) cell secretome released upon three\u2010dimensional (3D) culture. For the 2D\u2010cardiac hypoxia model, neonatal rat cardiomyocytes (CM) were cultured for 5 days at\u2009&lt;\u20091% (approaching anoxia) oxygen (O<jats:sub>2<\/jats:sub>) tension. Typical cardiac differentiation hallmarks and contractile ability were used to assess both the cardiomyocyte loss of functionality upon anoxia exposure and its possible recovery following the 5\u2010day\u2010treatment with SVF\u2010conditioned media (collected following 6\u2010day\u2010perfusion\u2010based culture on collagen scaffolds in either normoxia or approaching anoxia). The culture at\u2009&lt;\u20091% O\n<jats:sub>2<\/jats:sub> for 5 days mimicked the reversible condition of hibernating myocardium with still living and poorly contractile CM (reversible state). Only SVF\u2010medium conditioned in normoxia expressing a high level of the prosurvival hepatocyte\u2010growth factor (HGF) and insulin\u2010like growth factor (IGF) allowed the partial recovery of the functionality of damaged CM. The secretome generated by SVF\u2010engineered tissues showed a high paracrine potential to rescue the nonfunctional CM, therefore resulting in a promising patch\u2010based treatment of specific low\u2010perfused areas after myocardial infarction.<\/jats:p>","DOI":"10.1002\/bit.26824","type":"journal-article","created":{"date-parts":[[2018,9,1]],"date-time":"2018-09-01T06:54:52Z","timestamp":1535784892000},"page":"132-142","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Paracrine potential of adipose stromal vascular fraction cells to recover hypoxia\u2010induced loss of cardiomyocyte function"],"prefix":"10.1002","volume":"116","author":[{"given":"Myroslava","family":"Mytsyk","sequence":"first","affiliation":[{"name":"Department of Surgery University Hospital Basel Basel Switzerland"},{"name":"Department of Biomedicine University of Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Isu","sequence":"additional","affiliation":[{"name":"Department of Surgery University Hospital Basel Basel Switzerland"},{"name":"Department of Biomedicine University of Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Cerino","sequence":"additional","affiliation":[{"name":"Department of Surgery University Hospital Basel Basel Switzerland"},{"name":"Department of Biomedicine University of Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin T. 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