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PCC 6803 causes aberrant accumulation of glycogen. We previously tried to redirect the excess carbon stored as glycogen in the \n<jats:italic>cyabrB2<\/jats:italic>\u2010disrupted (\u2206\n<jats:italic>cyabrB2<\/jats:italic>) mutant by knockout of the \n<jats:italic>glgC<\/jats:italic> (slr1176) gene encoding glucose\u20101\u2010phosphate adenylyltransferase. However, complete knockout could not be attained, suggesting that accumulation of glycogen is essential for the \u0394\n<jats:italic>cyabrB2<\/jats:italic> mutant. In this study, we introduced the \n<jats:italic>cyabrB2<\/jats:italic> gene fused to the copper\u2010inducible \n<jats:italic>petE<\/jats:italic> promoter into the \u2206\n<jats:italic>cyabrB2<\/jats:italic> mutant. After complete knockout of \n<jats:italic>glgC<\/jats:italic> in the presence of copper, expression of P\n<jats:italic>petE<\/jats:italic>\u2010\n<jats:italic>cyabrB2<\/jats:italic> was turned off by copper removal to examine the effect of the double knockout of \n<jats:italic>cyabrB2<\/jats:italic> and \n<jats:italic>glgC<\/jats:italic>. Metabolome analysis and electron microscopic observation revealed that the double knockout causes a large decrease of sugar phosphates in glycolytic and oxidative pentose phosphate pathways and an increase of organic acids in the tricarboxylic acid cycle, amino acids and storage compounds such as polyhydroxybutyrate. When the ability of production of free fatty acids was conferred, synergetic positive effects of knockout of \n<jats:italic>cyabrB2<\/jats:italic> and \n<jats:italic>glgC<\/jats:italic> on productivity were observed by removal of both copper and nitrogen. The P\n<jats:italic>petE<\/jats:italic>\u2010\n<jats:italic>cyabrB2<\/jats:italic>\u0394\n<jats:italic>glgC<\/jats:italic> strain will further serve as a platform for studies on carbon allocation and metabolic engineering.<\/jats:p>","DOI":"10.1002\/bit.26842","type":"journal-article","created":{"date-parts":[[2018,9,25]],"date-time":"2018-09-25T13:23:31Z","timestamp":1537881811000},"page":"2974-2985","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effects of inactivation of the cyAbrB2 transcription factor together with glycogen synthesis on cellular metabolism and free fatty acid production in the cyanobacterium <i>Synechocystis<\/i> sp. PCC 6803"],"prefix":"10.1002","volume":"115","author":[{"given":"Yuta","family":"Kodama","sequence":"first","affiliation":[{"name":"Graduate School of Science and Engineering, Saitama University Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihito","family":"Kawahara","sequence":"additional","affiliation":[{"name":"Biological Science Research, KAO Corporation Wakayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuko","family":"Miyagi","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering, Saitama University Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiki","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering, Saitama University Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maki","family":"Kawai\u2010Yamada","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering, Saitama University Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuko","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering, Saitama University Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasushi","family":"Takimura","sequence":"additional","affiliation":[{"name":"Biological Science Research, KAO Corporation Wakayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1916-2053","authenticated-orcid":false,"given":"Yukako","family":"Hihara","sequence":"additional","affiliation":[{"name":"Graduate School of Science and Engineering, Saitama University Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,8]]},"reference":[{"key":"e_1_2_7_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00017837"},{"key":"e_1_2_7_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.12243"},{"key":"e_1_2_7_1_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2ee23181f"},{"key":"e_1_2_7_1_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.062950-0"},{"key":"e_1_2_7_1_6_1","first-page":"770","article-title":"The effects of dark incubation on cellular metabolism of the wild type cyanobacterium Synechocystis sp. 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