{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T16:01:39Z","timestamp":1782230499089,"version":"3.54.5"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,12,31]],"date-time":"2018-12-31T00:00:00Z","timestamp":1546214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100004319","name":"Pfizer","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004319","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Conjugated vaccines prepared from the capsular polysaccharide of <jats:italic>Streptococcus pneumoniae<\/jats:italic> can provide immunization against invasive pneumococcal disease, meningitis, and otitis media. One of the critical steps in the production of these vaccines is the removal of free (unreacted) polysaccharides from the protein\u2010polysaccharide conjugate. Experimental studies were performed to evaluate the effects of membrane pore size, filtrate flux, and solution conditions on the transmission of both the conjugate and free polysaccharide through different ultrafiltration membranes. Conjugate purification was done using diafiltration performed in a linearly\u2010scalable tangential flow filtration cassette. More than 98% of the free polysaccharide was removed within a 5\u2010diavolume diafiltration process, which is a significant improvement over previously reported results for purification of similar conjugated vaccines. These results clearly demonstrate the opportunities for using ultrafiltration\/diafiltration for the final purification of conjugated vaccine products.<\/jats:p>","DOI":"10.1002\/bit.26867","type":"journal-article","created":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T00:15:52Z","timestamp":1542586552000},"page":"591-597","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Purification of a conjugated polysaccharide vaccine using tangential flow diafiltration"],"prefix":"10.1002","volume":"116","author":[{"given":"Parinaz","family":"Emami","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering The Pennsylvania State University University Park  Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3768-789X","authenticated-orcid":false,"given":"Seyed Pouria","family":"Motevalian","sequence":"additional","affiliation":[{"name":"Biotherapeutics Pharmaceutical Sciences Pfizer Inc Andover Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin","family":"Pepin","sequence":"additional","affiliation":[{"name":"Biotherapeutics Pharmaceutical Sciences Pfizer Inc Andover Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1865-9156","authenticated-orcid":false,"given":"Andrew L.","family":"Zydney","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering The Pennsylvania State University University Park  Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,12,31]]},"reference":[{"key":"e_1_2_6_1_2_1","unstructured":"Anderson P. W. & Eby R. J. (1990).Immunogenic conjugates. US4902506A."},{"key":"e_1_2_6_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0008-6215(93)84085-K"},{"key":"e_1_2_6_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)00938-7"},{"key":"e_1_2_6_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.memsci.2018.05.024"},{"key":"e_1_2_6_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2012.06673.x"},{"key":"e_1_2_6_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.memsci.2015.04.047"},{"key":"e_1_2_6_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2016.06.095"},{"key":"e_1_2_6_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.2367"},{"key":"e_1_2_6_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.11.010"},{"key":"e_1_2_6_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-440585-1.50013-9"},{"key":"e_1_2_6_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.08.040"},{"key":"e_1_2_6_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cam.2010.03.019"},{"key":"e_1_2_6_1_14_1","first-page":"385","article-title":"Purification of Streptococcus pneumoniae capsular polysaccharides using aluminium phosphate and ethanol","volume":"6","author":"Macha C.","year":"2014","journal-title":"International Journal of Pharmacy and Pharmaceutical Sciences"},{"key":"e_1_2_6_1_15_1","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.1.3.399"},{"key":"e_1_2_6_1_16_1","unstructured":"McMaster R. (2000).Purification of polysaccharide\u2010protein conjugate vaccines by ultrafiltration with ammonium sulfate solutions. US 20140363463 A1."},{"key":"e_1_2_6_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0376-7388(99)00111-8"},{"key":"e_1_2_6_1_18_1","unstructured":"Shin J.\u2010H. Yang J.\u2010H. Ham D.\u2010S. Park M.\u2010H. Kim H. Noh M.\u2010J. \u2026Yang S.\u2010Y.(2015).Polyvalent pneumococcal polysaccharide\u2010protein conjugate composition.US20150190520 A1."},{"key":"e_1_2_6_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.synbio.2016.12.002"},{"key":"e_1_2_6_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0376-7388(96)00319-5"},{"key":"e_1_2_6_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.memsci.2005.02.024"},{"key":"e_1_2_6_1_22_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114858"},{"key":"e_1_2_6_1_23_1","volume-title":"Ultrafiltration and microfiltration: principles and applications","author":"Zeman L. J.","year":"1996"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbit.26867","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.26867","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bit.26867","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.26867","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T22:12:47Z","timestamp":1760047967000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.26867"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,31]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1002\/bit.26867"],"URL":"https:\/\/doi.org\/10.1002\/bit.26867","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,12,31]]},"assertion":[{"value":"2018-07-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}