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Covalent modification of enzymes with polymers has led to polymer\u2013enzyme conjugates (PECs) that are soluble in organic solvents, but the process is quite elaborate. Poly(2\u2010oxazoline)s (POx) with the end group 2,2\u2032\u2010imino diacetic acid were shown to form reversible, nano\u2010sized noncovalent aggregates with enzymes. These PECs give clear solutions in organic solvents. The enzymes lysozyme, horseradish peroxidase (HRP), laccase, \u03b1\u2010chymotrypsin (CT), catalase, and alcohol dehydrogenase could be solubilized in chloroform and toluene at concentrations of up to 2\u2009mg protein\/ml. Laccase, HRP, and CT were shown to survive the transfer into the organic medium and back to water in their active form. The distribution coefficient of the proteins between water and the organic solvent was shown to be dependent on the nature of the POx backbone. All three biocatalysts exhibit greatly enhanced activity in the respective organic solvent.<\/jats:p>","DOI":"10.1002\/bit.26877","type":"journal-article","created":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T09:34:44Z","timestamp":1543224884000},"page":"272-282","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Poly(2\u2010oxazoline)s terminated with 2,2\u2032\u2010imino diacetic acid form noncovalent polymer\u2013enzyme conjugates that are highly active in organic solvents"],"prefix":"10.1002","volume":"116","author":[{"given":"Montasser","family":"Hijazi","sequence":"first","affiliation":[{"name":"Department of Bio and Chemical Engineering TU Dortmund Dortmund Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pia","family":"Spiekermann","sequence":"additional","affiliation":[{"name":"Department of Bio and Chemical Engineering TU Dortmund Dortmund Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Krumm","sequence":"additional","affiliation":[{"name":"Department of Bio and Chemical Engineering TU Dortmund Dortmund Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1531-0795","authenticated-orcid":false,"given":"Joerg C.","family":"Tiller","sequence":"additional","affiliation":[{"name":"Department of Bio and Chemical Engineering TU Dortmund Dortmund Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,12,14]]},"reference":[{"key":"e_1_2_6_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(02)75364-2"},{"key":"e_1_2_6_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00792-012-0498-0"},{"key":"e_1_2_6_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(81)90002-8"},{"key":"e_1_2_6_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2004.02.003"},{"key":"e_1_2_6_1_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm801162e"},{"key":"e_1_2_6_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21868"},{"key":"e_1_2_6_1_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/ma052592h"},{"key":"e_1_2_6_1_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/ol005593x"},{"key":"e_1_2_6_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.20243"},{"key":"e_1_2_6_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.200600452"},{"key":"e_1_2_6_1_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsmacrolett.6b00137"},{"key":"e_1_2_6_1_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm1015877"},{"key":"e_1_2_6_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymer.2011.12.027"},{"key":"e_1_2_6_1_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1988.tb25834.x"},{"key":"e_1_2_6_1_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm701281x"},{"key":"e_1_2_6_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(89)90094-X"},{"key":"e_1_2_6_1_19_1","doi-asserted-by":"publisher","DOI":"10.1049\/ip-nbt:20050005"},{"key":"e_1_2_6_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9861(66)81058-5"},{"key":"e_1_2_6_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymer.2015.04.010"},{"key":"e_1_2_6_1_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01024634"},{"key":"e_1_2_6_1_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340030208"},{"key":"e_1_2_6_1_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/la403598s"},{"key":"e_1_2_6_1_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1992.tb19823.x"},{"key":"e_1_2_6_1_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac060634"},{"key":"e_1_2_6_1_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-006-0680-2"},{"key":"e_1_2_6_1_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201800168"},{"key":"e_1_2_6_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7799(86)90244-1"},{"key":"e_1_2_6_1_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/cctc.201300841"},{"key":"e_1_2_6_1_31_1","unstructured":"Keesey J.(1987). 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