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To reduce downstream cost associated with continuous centrifugation and salt containing wastewater treatment,\n                    <jats:italic>Halomonas campaniensis<\/jats:italic>\n                    strain LS21 was engineered to become self\u2010flocculating by knocking out an\n                    <jats:italic>etf<\/jats:italic>\n                    operon encoding two subunits of an electron transferring flavoprotein in the predicted electron transfer chain. Self\u2010flocculation could be attributed to the decrease of the surface charge and increase of the cellular hydrophobicity resulted from deleted\n                    <jats:italic>etf<\/jats:italic>\n                    . A wastewaterless fermentation strategy based on the self\u2010flocculating\n                    <jats:italic>H. campaniensis<\/jats:italic>\n                    was developed for growth and the production of poly\u20103\u2010hydroxybutyrate (PHB) as an example. Most microbial cells flocculated and precipitated to the bottom of the bioreactor within 1\u2009min after stopping the aeration and agitation. The supernatant can be used again without sterilization or inoculation for the growth of the next batch after collecting the precipitated cell mass. The wastewaterless process was conducted for four runs without generating wastewater. PHB accumulation by the self\u2010flocculent strain was enhanced via promoter and ribosome binding site optimizations, the productivities of cell dry weight and PHB were increased from 0.45 and 0.18\u2009g\u00b7L\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u00b7hr\n                    <jats:sup>\u22121<\/jats:sup>\n                    for the batch process compared to 0.82 and 0.33\u2009g\u00b7L\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u00b7hr\n                    <jats:sup>\u22121<\/jats:sup>\n                    for the wastewaterless continuous process, respectively. This has clearly demonstrated the advantages of the wastewaterless process in that it not only reduces wastewater but also increases cell growth and product formation efficiency in a given period of time.\n                  <\/jats:p>","DOI":"10.1002\/bit.26897","type":"journal-article","created":{"date-parts":[[2018,12,8]],"date-time":"2018-12-08T07:39:01Z","timestamp":1544254741000},"page":"805-815","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["Engineering self\u2010flocculating\n                    <i>Halomonas campaniensis<\/i>\n                    for wastewaterless open and continuous fermentation"],"prefix":"10.1002","volume":"116","author":[{"given":"Chen","family":"Ling","sequence":"first","affiliation":[{"name":"Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University Beijing 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