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However, competition for hydrogen (H<jats:sub>2<\/jats:sub>), the electron\u2010donor substrate, may cause poor dechlorination when multiple electron acceptors are present. Common acceptors in anaerobic environments are nitrate (NO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>), sulfate (SO<jats:sub>4<\/jats:sub><jats:sup>2\u2212<\/jats:sup>), and bicarbonate (HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>). We evaluated CF dechlorination in the presence of HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> at 1.56 e<jats:sup>\u2212<\/jats:sup> Eq\/m<jats:sup>2<\/jats:sup>\u2010day, then NO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> at 0.04\u20130.15 e<jats:sup>\u2212<\/jats:sup> Eq\/m<jats:sup>2<\/jats:sup>\u2010day, and finally NO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> (0.04 e<jats:sup>\u2212<\/jats:sup> Eq\/m<jats:sup>2<\/jats:sup>\u2010day) along with SO<jats:sub>4<\/jats:sub><jats:sup>2\u2212<\/jats:sup> at 0.33 e<jats:sup>\u2212<\/jats:sup> Eq\/m<jats:sup>2<\/jats:sup>\u2010day in an H<jats:sub>2<\/jats:sub>\u2010based membrane biofilm reactor (MBfR). When the biofilm was initiated with CF\u2010dechlorination conditions (no NO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> or SO<jats:sub>4<\/jats:sub><jats:sup>2\u2212<\/jats:sup>), it yielded a CF flux of 0.14 e<jats:sup>\u2212<\/jats:sup> Eq\/m<jats:sup>2<\/jats:sup>\u2010day and acetate production via homoacetogenesis up to 0.26 e<jats:sup>\u2212<\/jats:sup> eq\/m<jats:sup>2<\/jats:sup>\u2010day. Subsequent addition of NO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> at 0.05 e<jats:sup>\u2212<\/jats:sup> Eq\/m<jats:sup>2<\/jats:sup>\u2010day maintained full CF dechlorination and homoacetogenesis, but NO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> input at 0.15 e<jats:sup>\u2212<\/jats:sup> Eq\/m<jats:sup>2<\/jats:sup>\u2010day caused CF to remain in the reactor's effluent and led to negligible acetate production. The addition of SO<jats:sub>4<\/jats:sub><jats:sup>2\u2212<\/jats:sup> did not affect CF reduction, but SO<jats:sub>4<\/jats:sub><jats:sup>2\u2212<\/jats:sup> reduction significantly altered the microbial community by introducing sulfate\u2010reducing <jats:italic>Desulfovibrio<\/jats:italic> and more sulfur\u2010oxidizing <jats:italic>Arcobacter<\/jats:italic>. <jats:italic>Dechloromonas<\/jats:italic> appeared to carry out CF dechlorination and denitrification, whereas <jats:italic>Acetobacterium<\/jats:italic> (homoacetogen)\u00a0may have been involved with hydrolytic dechlorination. Modifications to the electron acceptors fed to the\u00a0MBfR caused the microbial community to undergo changes in structure that reflected changes in the removal fluxes.<\/jats:p>","DOI":"10.1002\/bit.26945","type":"journal-article","created":{"date-parts":[[2019,2,3]],"date-time":"2019-02-03T07:09:40Z","timestamp":1549177780000},"page":"1439-1448","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Electron\u2010acceptor loadings affect chloroform dechlorination in a hydrogen\u2010based membrane biofilm reactor"],"prefix":"10.1002","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7611-8441","authenticated-orcid":false,"given":"YenJung Sean","family":"Lai","sequence":"first","affiliation":[{"name":"School of Sustainable Engineering and the Built Environment Arizona State University, Biodesign Institute Tempe 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