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It is desirable to use the adsorption\u2010based chromatographic separations that so effectively underpin the therapeutic protein manufacture. However fundamental differences in the size and stability of this class of product mean it is necessary to revisit the design of sorbent's morphology and surface chemistry. In this study, the behaviour of a cellulose nanofiber ion\u2010exchange sorbent derivatised with quaternary amine ligands at defined densities is characterised to address this. This material was selected as it has a large accessible surface area for viral particles and rapid process times. Initially, the impact of surface chemistry on infective product recovery using low (440\u2009\u00b5mol\/g), medium (750\u2009\u00b5mol\/g), and high (1029\u2009\u00b5mol\/g) ligand densities is studied. At higher densities product stability is reduced, this effect increased with prolonged adsorption durations of 24\u2009min with just ~10% loss at low ligand density versus ~50% at high. This could be mitigated by using a high flow rate to reduce the cycle time to ~1\u2009min. Next, the impact of ligand density on the separation's resolution was evaluated. Key to understanding virus quality is the virus particle: infectious virus particle ratio. It was found this parameter could be manipulated using ligand density and elution strategy. Together this provides a basis for viral vector separations that allows for their typically low titres and labile nature by using high liquid velocity to minimise both load and on\u2010column times while separating key product and process\u2010related impurities.<\/jats:p>","DOI":"10.1002\/bit.26972","type":"journal-article","created":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T01:51:09Z","timestamp":1552960269000},"page":"1698-1709","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Adenovirus 5 recovery using nanofiber ion\u2010exchange adsorbents"],"prefix":"10.1002","volume":"116","author":[{"given":"Jordan","family":"Turnbull","sequence":"first","affiliation":[{"name":"Department of Biochemical Engineering University College London London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernice","family":"Wright","sequence":"additional","affiliation":[{"name":"Department of 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