{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:06:05Z","timestamp":1790251565881,"version":"4.1.0"},"reference-count":69,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,6,20]],"date-time":"2019-06-20T00:00:00Z","timestamp":1560988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000971","name":"Amyotrophic Lateral Sclerosis Association","doi-asserted-by":"publisher","award":["15\u2010IIP\u2010201"],"award-info":[{"award-number":["15\u2010IIP\u2010201"]}],"id":[{"id":"10.13039\/100000971","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011089","name":"University of Wisconsin Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011089","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007015","name":"University of Wisconsin-Madison","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007015","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent advances in bioengineering have enabled cell culture systems that more closely mimic the native cellular environment. Here, we demonstrated that human induced pluripotent stem cell (iPSC)\u2010derived myogenic progenitors formed highly\u2010aligned myotubes and contracted when seeded on two\u2010dimensional micropatterned platforms. The differentiated cells showed clear nuclear alignment and formed elongated myotubes dependent on the width of the micropatterned lanes. Topographical cues from micropatterning and physiological substrate stiffness improved the formation of well\u2010aligned and multinucleated myotubes similar to myofibers. These aligned myotubes exhibited spontaneous contractions specifically along the long axis of the pattern. Notably, the micropatterned platforms developed bundle\u2010like myotubes using patient\u2010derived iPSCs with a background of Pompe disease (glycogen storage disease type II) and even enhanced the disease phenotype as shown through the specific pathology of abnormal lysosome accumulations. A highly\u2010aligned formation of matured myotubes holds great potential in further understanding the process of human muscle development, as well as advancing in vitro pharmacological studies for skeletal muscle diseases.<\/jats:p>","DOI":"10.1002\/bit.27075","type":"journal-article","created":{"date-parts":[[2019,5,27]],"date-time":"2019-05-27T06:12:39Z","timestamp":1558937559000},"page":"2377-2392","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Micropatterned substrates with physiological stiffness promote cell maturation and Pompe disease phenotype in human induced pluripotent stem cell\u2010derived skeletal myocytes"],"prefix":"10.1002","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5147-2900","authenticated-orcid":false,"given":"Nunnapas","family":"Jiwlawat","sequence":"first","affiliation":[{"name":"Department of Comparative Biosciences University of Wisconsin Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eileen M.","family":"Lynch","sequence":"additional","affiliation":[{"name":"Department of Comparative Biosciences University of Wisconsin Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett N.","family":"Napiwocki","sequence":"additional","affiliation":[{"name":"Wisconsin Institute for Discovery University of Wisconsin Madison Wisconsin"},{"name":"Department of Biomedical Engineering University of Wisconsin Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alana","family":"Stempien","sequence":"additional","affiliation":[{"name":"Wisconsin Institute for Discovery University of Wisconsin Madison Wisconsin"},{"name":"Department of Biomedical Engineering University of Wisconsin Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randolph S.","family":"Ashton","sequence":"additional","affiliation":[{"name":"Wisconsin Institute for Discovery University of Wisconsin Madison Wisconsin"},{"name":"Department of Biomedical Engineering University of Wisconsin Madison Wisconsin"},{"name":"The Stem Cell and Regenerative Medicine Center University of Wisconsin Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy J.","family":"Kamp","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Wisconsin Madison Wisconsin"},{"name":"The Stem Cell and Regenerative Medicine Center University of Wisconsin Madison Wisconsin"},{"name":"Department of Cell and Regenerative Biology University of Wisconsin Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy C.","family":"Crone","sequence":"additional","affiliation":[{"name":"Wisconsin Institute for Discovery University of Wisconsin Madison Wisconsin"},{"name":"Department of Biomedical Engineering University of Wisconsin Madison Wisconsin"},{"name":"The Stem Cell and Regenerative Medicine Center University of Wisconsin Madison Wisconsin"},{"name":"Department of Engineering Physics University of Wisconsin Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3253-0711","authenticated-orcid":false,"given":"Masatoshi","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Comparative Biosciences University of Wisconsin Madison Wisconsin"},{"name":"Department of Biomedical Engineering University of Wisconsin Madison Wisconsin"},{"name":"The Stem Cell and Regenerative Medicine Center University of Wisconsin Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,6,20]]},"reference":[{"issue":"2","key":"e_1_2_8_1_2_1","doi-asserted-by":"crossref","first-page":"149","DOI":"10.5966\/sctm.2013-0095","article-title":"Myogenic differentiation of muscular dystrophy\u2010specific induced pluripotent stem cells for use in drug discovery","volume":"3","author":"Abujarour R.","year":"2014","journal-title":"Stem Cells Translational Medicine"},{"issue":"30","key":"e_1_2_8_1_3_1","doi-asserted-by":"crossref","first-page":"3738","DOI":"10.1002\/adfm.201203319","article-title":"Micropatterning alginate substrates for in vitro cardiovascular muscle on a chip","volume":"23","author":"Agarwal A.","year":"2013","journal-title":"Advanced Functional Materials"},{"key":"e_1_2_8_1_4_1","first-page":"19","volume-title":"Science of flexibility","author":"Alter J. 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