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Especially, the microfluidic platform of a DNA curtain is highly suitable for a biochip. In the DNA curtain, DNA molecules are aligned along chromium nanobarriers, which are fabricated on a slide surface, and visualized using an intercalating dye, YOYO\u20101. Although the chromium barriers confer precise geometric alignment of DNA, reuse of the slides is limited by wear of the barriers during cleaning. YOYO\u20101 is rapidly photobleached and causes photocleavage of DNA under continuous laser illumination, restricting DNA observation to a brief time window. To address these challenges, we developed a new nanopatterned slide, upon which carved nanotrenches serve as diffusion barriers. The nanotrenches were robust under harsh cleaning conditions, facilitating the maintenance of surface cleanliness that is essential to slide reuse. We also stained DNA with a fluorescent protein with a DNA\u2010binding motif, fluorescent protein\u2013DNA binding peptide (FP\u2013DBP). FP\u2013DBP was slowly photobleached and did not cause DNA photocleavage. This new DNA curtain system enables a more stable and repeatable investigation of real\u2010time protein\u2013DNA interactions and will serve as a good platform for lab\u2010on\u2010a\u2010chip.<\/jats:p>","DOI":"10.1002\/bit.27331","type":"journal-article","created":{"date-parts":[[2020,3,12]],"date-time":"2020-03-12T06:28:35Z","timestamp":1583994515000},"page":"1640-1648","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["High\u2010throughput single\u2010molecule imaging system using nanofabricated trenches and fluorescent DNA\u2010binding proteins"],"prefix":"10.1002","volume":"117","author":[{"given":"Yujin","family":"Kang","sequence":"first","affiliation":[{"name":"School of Life Sciences Ulsan National Institute of Science and Technology  Ulsan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na Young","family":"Cheon","sequence":"additional","affiliation":[{"name":"School of Life Sciences Ulsan National Institute of Science and Technology  Ulsan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jongjin","family":"Cha","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayoung","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Life Sciences Ulsan National Institute of Science and Technology  Ulsan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyung\u2010il","family":"Kim","sequence":"additional","affiliation":[{"name":"UNIST Central Research Facilities (UCRF) Ulsan National Institute of Science and Technology  Ulsan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luda","family":"Lee","sequence":"additional","affiliation":[{"name":"UNIST Central Research Facilities (UCRF) Ulsan National 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