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To that end, there has been an interest in designing immunofocusing strategies to direct the immune response to specific, conserved regions on antigenic proteins. Modulation of glycosylation is one such immunofocusing strategy; extensive glycosylation is often exploited by pathogens for immune evasion. Masking epitopes on protein immunogens with \u201cself\u201d glycans can also shield the underlying protein surface from humoral immune surveillance. We review recent advances in applying glycosylation as an immunofocusing tool. We also highlight recent interesting work in the HIV\u20101 field involving the identification and elicitation of broadly neutralizing antibodies that incorporate glycans into their binding epitopes.<\/jats:p>","DOI":"10.1002\/bit.27361","type":"journal-article","created":{"date-parts":[[2020,4,24]],"date-time":"2020-04-24T12:23:20Z","timestamp":1587731000000},"page":"2556-2570","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Glycosylation as a tool for rational vaccine design"],"prefix":"10.1002","volume":"117","author":[{"given":"Vivek","family":"Hariharan","sequence":"first","affiliation":[{"name":"School of Chemical &amp; Biomolecular Engineering Georgia Institute of Technology  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3084-4098","authenticated-orcid":false,"given":"Ravi 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