{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:19:40Z","timestamp":1790839180439,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T00:00:00Z","timestamp":1594252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["1R15EY027111"],"award-info":[{"award-number":["1R15EY027111"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glaucoma is a degenerative eye disease in which damage to the optic nerve leads to a characteristic loss of vision. The primary risk factor for glaucoma is an increased intraocular pressure that is caused by an imbalance of aqueous humor generation and subsequent drainage through the trabecular meshwork (TM) drainage system. The small size, donor tissue limitations, and high complexity of the TM make it difficult to research the relationship between the TM cells and their immediate environment. Thus, a biomaterial\u2010based approach may be more appropriate for research manipulations and in vitro drug development platforms. In this work, human TM (hTM) cells were cultured on various collagen scaffolds containing different glycosaminoglycans (GAGs) and different pore architectures to better understand how hTM cells respond to changes in their extracellular environment. Cellular response was measured by quantifying cellular proliferation and expression of an important extracellular matrix protein, fibronectin. The pore architecture of the scaffolds was altered using freeze\u2010casting technique to make both large and small pores that were aligned or with a non\u2010aligned random structure. The composition of the scaffolds was altered with the addition of chondroitin sulfate and\/or hyaluronic acid. It was found that the hTM cells grown on large pore scaffolds proliferate more than those grown on small pores. There was an increase in the fibronectin expression with the incorporation of GAGs, and its morphology was changed by the underlying pore architecture. This work will help provide an insight into the behavior of hTM cells when introducing changes in their microenvironment.<\/jats:p>","DOI":"10.1002\/bit.27477","type":"journal-article","created":{"date-parts":[[2020,6,27]],"date-time":"2020-06-27T05:35:44Z","timestamp":1593236144000},"page":"3150-3159","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Human trabecular meshwork cell behavior is influenced by collagen scaffold pore architecture and glycosaminoglycan composition"],"prefix":"10.1002","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4778-9067","authenticated-orcid":false,"given":"Matthew J.","family":"Osmond","sequence":"first","affiliation":[{"name":"Department of Chemical &amp; Biological Engineering Colorado School of Mines  Golden Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5170-7116","authenticated-orcid":false,"given":"Melissa D.","family":"Krebs","sequence":"additional","affiliation":[{"name":"Department of Chemical &amp; Biological Engineering Colorado School of Mines  Golden Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7452-4523","authenticated-orcid":false,"given":"Mina B.","family":"Pantcheva","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology University of Colorado School of Medicine  Aurora Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,9]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"1699","article-title":"Current primary open\u2010angle glaucoma treatments and future directions","volume":"6","author":"Beidoe G.","year":"2012","journal-title":"Clinical Ophthalmology"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(62)90095-7"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2017.02.006"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.04.021"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0144-8617(03)00144-9"},{"key":"e_1_2_7_7_1","first-page":"14","article-title":"Glucocorticoid\u2010Induced formation of cross\u2010linked actin networks in cultured human trabecular meshwork cells","volume":"35","author":"Clark A. 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