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In this study, an adenoviral vector carrying the transforming growth factor\u2010\u03b23 (TGF\u2010\u03b23) gene was constructed and introduced into dedifferentiated chondrocytes, which were then cocultured with ATDC5 cells in an alginate hydrogel system. The results showed that the experimental groups exhibited better cell viability and higher levels of cartilage\u2010related genes than the control groups. In this coculture system, the chondrogenic differentiation of ATDC5 cells was effectively induced by TGF\u2010\u03b23 and other latent cytokines that were produced by the transfected chondrocytes. Thus, this method can avoid the degradation of exogenous TGF\u2010\u03b23, and it can protect ATDC5 cells during virus transfection to maintain cell viability and chondrogenic differentiation capability. Taken together, this study provides fresh insights for applying this genetically manipulated coculture system to cartilage repair in the future.<\/jats:p>","DOI":"10.1002\/bit.27482","type":"journal-article","created":{"date-parts":[[2020,7,7]],"date-time":"2020-07-07T10:50:09Z","timestamp":1594119009000},"page":"3173-3181","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Enhanced chondrogenesis in a coculture system with genetically manipulated dedifferentiated chondrocytes and ATDC5 cells"],"prefix":"10.1002","volume":"117","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4338-2606","authenticated-orcid":false,"given":"Yongchang","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Joint Surgery The First Affiliated Hospital of Guangzhou Medical University  Guangzhou China"},{"name":"Guangdong Key Laboratory of Orthopaedic Technology and Implant Materials  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingshuai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Joint Surgery The First Affiliated Hospital of Guangzhou Medical University  Guangzhou China"},{"name":"Department of Orthopaedics Dongguan Tungwah Hospital  Dongguan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanzheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Joint Surgery The First Affiliated Hospital of Guangzhou Medical University  Guangzhou China"},{"name":"Guangdong Key Laboratory of Orthopaedic Technology and Implant Materials  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shicong","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Joint Surgery The First Affiliated Hospital of Guangzhou Medical University  Guangzhou China"},{"name":"Guangdong Key Laboratory of Orthopaedic Technology and Implant Materials  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Joint Surgery The First Affiliated Hospital of Guangzhou Medical University  Guangzhou China"},{"name":"Guangdong Key Laboratory of Orthopaedic Technology and Implant Materials  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Joint Surgery The First Affiliated Hospital of Guangzhou Medical University  Guangzhou China"},{"name":"Guangdong Key Laboratory of Orthopaedic Technology and Implant Materials  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,14]]},"reference":[{"issue":"6","key":"e_1_2_7_2_1","first-page":"669","article-title":"Mesenchymal stem cell\u2010based tissue engineering strategies for repair of articular cartilage","volume":"29","author":"Ahmed T. 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