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Unfortunately, this treatment option is not available universally due to the serious shortage of donors. Thus, alternative methods have been developed that are aimed at prolonging the life of patients, including hepatic cells transplantation and bridging therapy based on hybrid bioartificial liver devices. Parenchymal liver cells are highly differentiated and perform many complex functions, such as detoxification and protein synthesis. Unfortunately, isolated hepatocytes display a rapid decline in viability and liver\u2010specific functions. A number of methods have been developed to maintain hepatocytes in their highly differentiated state in vitro, amongst them the most promising being 3D growth scaffolds and decellularized tissues or coculture with other cell types required for the heterotypic cell\u2010cell interactions. Here we present a novel approach to the hepatic cells culture based on the feeder layer cells genetically modified using lentiviral vector to stably produce additional amounts of hepatocyte growth factor and show the positive influence of these coculture conditions on the preservation of the hepatic functions of the liver parenchymal cells' model\u2014C3A cells.<\/jats:p>","DOI":"10.1002\/bit.27551","type":"journal-article","created":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T10:54:41Z","timestamp":1599130481000},"page":"72-81","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of genetically modified human skin fibroblasts, stably overexpressing hepatocyte growth factor, on hepatic functions of cocultured C3A cells"],"prefix":"10.1002","volume":"118","author":[{"given":"Agnieszka","family":"Wencel","sequence":"first","affiliation":[{"name":"Nalecz Institute of Biocybernetics and Biomedical Engineering Polish Academy of Sciences Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malgorzata","family":"Ciezkowska","sequence":"additional","affiliation":[{"name":"Nalecz Institute of Biocybernetics and Biomedical Engineering Polish Academy of Sciences Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Wisniewska","sequence":"additional","affiliation":[{"name":"Nalecz Institute of Biocybernetics and Biomedical Engineering Polish Academy of Sciences Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karolina E.","family":"Zakrzewska","sequence":"additional","affiliation":[{"name":"Nalecz Institute of Biocybernetics and Biomedical Engineering Polish Academy of Sciences Warsaw Poland"},{"name":"Institute of Fundamental Technological Research Polish Academy of Sciences Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorota G.","family":"Pijanowska","sequence":"additional","affiliation":[{"name":"Nalecz Institute of Biocybernetics and Biomedical Engineering Polish Academy of Sciences Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1706-2788","authenticated-orcid":false,"given":"Krzysztof D.","family":"Pluta","sequence":"additional","affiliation":[{"name":"Nalecz Institute of Biocybernetics and Biomedical Engineering Polish Academy of Sciences Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1517\/17425255.2012.685159"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/microarrays4010064"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx015518a"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2014.10.016"},{"issue":"1","key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"25187","DOI":"10.1038\/srep25187","article-title":"Characterization of primary human hepatocyte spheroids as a model system for drug\u2010induced liver injury, liver function and disease","volume":"6","author":"Bell C. 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