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This toolbox facilitates surface functionalization either as a one\u2010 or a two\u2010step strategy. In the case of the one\u2010step strategy, the desired functionality was directly introduced to LCI. For the two\u2010step strategy, LCI was modified with a reactive group, which can be further functionalized (e.g., employing \u201cclick\u201d chemistry). Sortagging of LCI, employing sortase A from <jats:italic>Staphylococcus aureus<\/jats:italic>, was achieved with six different amine compounds: dibenzocyclooctyne amine, biotin\u2010polyethylene glycol amine, Cyanine\u20103 amine, kanamycin, methoxypolyethylene glycol amine (Mn\u2009=\u20095000\u2009Da), and 2,2,3,3,4,4,4\u2010Heptafluorobutylamine. The purification of LCI\u2010amine sortagging products was performed by a negative purification using <jats:italic>Strep<\/jats:italic>\u2010tag II affinity chromatography, resulting in LCI\u2010amine conjugates with purities &gt;90%. For the two\u2010step strategy, the LCI\u2010dibenzocyclooctyne sortagging product was purified and enabled, through copper\u2010free azide\u2013alkyne \u201cclick\u201d chemistry, universal surface functionalization of material surfaces such as polypropylene, polyethylene terephthalate, stainless steel, gold, and silicon. The click reaction was performed before or after surface binding of LCI\u2010dibenzocyclooctyne. Finally, in the case of the one\u2010step strategy, polypropylene was directly functionalized with Cyanine\u20103 and biotin\u2010polyethylene glycol amine.<\/jats:p>","DOI":"10.1002\/bit.27666","type":"journal-article","created":{"date-parts":[[2021,1,7]],"date-time":"2021-01-07T14:17:42Z","timestamp":1610029062000},"page":"1520-1530","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["A peptide\u2010based coating toolbox to enable click chemistry on polymers, metals, and silicon through sortagging"],"prefix":"10.1002","volume":"118","author":[{"given":"Maximilian","family":"N\u00f6th","sequence":"first","affiliation":[{"name":"Lehrstuhl f\u00fcr Biotechnologie RWTH Aachen University Aachen Germany"},{"name":"DWI \u2013 Leibniz Institute for Interactive Materials Aachen 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