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This adherent cell culture platform requires the use of surfaces to support cell growth, typically roller bottles, or microcarriers. We have recently compared the production of rVSV\u2010ZEBOV on Vero cells between microcarrier and fixed\u2010bed bioreactors. However, suspension cultures are considered superior with regard to process scalability. Therefore, we further explore the Vero suspension system for recombinant vesicular stomatitis virus (rVSV)\u2010vectored vaccine production. Previously, this suspension cell line was only able to be cultivated in a proprietary medium. Here, we expand the adaptation and bioreactor cultivation to a serum\u2010free commercial medium. Following small\u2010scale optimization and screening studies, we demonstrate bioreactor productions of highly relevant vaccines and vaccine candidates against Ebola virus disease, HIV, and coronavirus disease 2019 in the Vero suspension system. rVSV\u2010ZEBOV, rVSV\u2010HIV, and rVSV<jats:sub>Ind<\/jats:sub>\u2010<jats:italic>msp<\/jats:italic>\u2010S<jats:sub>F<\/jats:sub>\u2010<jats:italic>Gtc<\/jats:italic> can replicate to high titers in the bioreactor, reaching 3.87\u2009\u00d7\u200910<jats:sup>7<\/jats:sup> TCID<jats:sub>50<\/jats:sub>\/ml, 2.12\u2009\u00d7\u200910<jats:sup>7<\/jats:sup> TCID<jats:sub>50<\/jats:sub>\/ml, and 3.59\u2009\u00d7\u200910<jats:sup>9<\/jats:sup> TCID<jats:sub>50<\/jats:sub>\/ml, respectively. Furthermore, we compare cell\u2010specific productivities, and the quality of the produced viruses by determining the ratio of total viral particles to infectious viral particles.<\/jats:p>","DOI":"10.1002\/bit.27785","type":"journal-article","created":{"date-parts":[[2021,4,10]],"date-time":"2021-04-10T07:35:13Z","timestamp":1618040113000},"page":"2649-2659","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Bioreactor production of rVSV\u2010based vectors in Vero cell suspension cultures"],"prefix":"10.1002","volume":"118","author":[{"given":"Sascha","family":"Kiesslich","sequence":"first","affiliation":[{"name":"Department of Bioengineering McGill University Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyoung N.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry The University of Western Ontario London Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun F.","family":"Shen","sequence":"additional","affiliation":[{"name":"Human Health Therapeutics Research Center National Research Council of Canada Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Yong","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Schulich School of Medicine and Dentistry The University of Western Ontario London Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amine A.","family":"Kamen","sequence":"additional","affiliation":[{"name":"Department of Bioengineering McGill University Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)31604-4"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mex.2020.100806"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2019.09.044"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)32621-6"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.02.052"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2020.107608"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2020.01.015"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00222-15"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2012.01812.x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2004.03.003"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-7506-0421-5.50096-9"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2020.10.058"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004731"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvacx.2019.100009"},{"key":"e_1_2_10_16_1","first-page":"1","article-title":"A tool with many applications: Vesicular stomatitis virus in research and medicine","volume":"00","author":"Munis A. 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