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In this study, recombinant S protein of SARS\u2010CoV\u20102 was expressed in <jats:italic>Rachiplusia nu<\/jats:italic>, an important agronomic plague. One gram of insect larvae produces an amount of S protein sufficient for 150 determinations in the ELISA method herein developed. We established a rapid production process for SARS\u2010CoV\u20102 S protein that showed immunoreactivity for anti\u2010SARS\u2010CoV\u20102 antibodies and was used as a single antigen for developing the ELISA method with high sensitivity (96.2%) and specificity (98.8%). Our findings provide an efficient and cost\u2010effective platform for large\u2010scale S protein production, and the scale\u2010up is linear, thus avoiding the use of complex equipment like bioreactors.<\/jats:p>","DOI":"10.1002\/bit.27889","type":"journal-article","created":{"date-parts":[[2021,7,15]],"date-time":"2021-07-15T09:24:20Z","timestamp":1626341060000},"page":"4129-4137","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Rapid and cost\u2010effective process based on insect larvae for scale\u2010up production of SARS\u2010COV\u20102 spike protein for serological COVID\u201019 testing"],"prefix":"10.1002","volume":"118","author":[{"given":"Ignacio","family":"Smith","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Inmunolog\u00eda, Biotecnolog\u00eda y Gen\u00e9tica, Facultad de Farmacia y Bioqu\u00edmica, C\u00e1tedra de Biotecnologia Universidad de Buenos Aires  Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Universidad de Buenos Aires Instituto de Nanobiotecnolog\u00eda (NANOBIOTEC)  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregorio Juan","family":"Mc Callum","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Inmunolog\u00eda, Biotecnolog\u00eda y Gen\u00e9tica, Facultad de Farmacia y Bioqu\u00edmica, C\u00e1tedra de Biotecnologia Universidad de Buenos Aires  Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Universidad de Buenos Aires Instituto de Nanobiotecnolog\u00eda (NANOBIOTEC)  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana Victoria","family":"Sabljic","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Inmunolog\u00eda, Biotecnolog\u00eda y Gen\u00e9tica, Facultad de Farmacia y Bioqu\u00edmica, C\u00e1tedra de Inmunolog\u00eda Universidad de Buenos Aires  Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Universidad de Buenos Aires Instituto de Estudios de la Inmunidad Humoral \u201cProf. Ricardo A. 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