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Our data here paint a different picture: large\u2010scale exchange of cellular material through EVs. To visualize the dynamic production and cellular uptake of EVs, we used correlative confocal microscopy and scanning electron microscopy, as well as flow cytometry to interrogate labeled cells. Using cells expressing fluorescent proteins (GFP, miRFP703) and cells tagged with protein and RNA dyes, we show that Chinese hamster ovary (CHO) cells dynamically produce and uptake EVs to exchange proteins and RNAs at a large scale. Applying a simple model to our data, we estimate, for the first time, the per cell\u2010specific rates of EV production (68 and 203 microparticles and exosomes, respectively, per day). This EV\u2010mediated massive exchange of cellular material observed in CHO cultures was also observed in cultured human CHRF\u2010288\u201011 and primary hematopoietic stem and progenitor cells. This study demonstrates an underappreciated massive protein and RNA exchange between cells mediated by EVs spanning cell type, suggesting that the proximity of cells in normal and tumor tissues may also result in prolific exchange of cellular material. This exchange would be expected to homogenize the cell\u2010population cytosol and dynamically regulate cell proliferation and the cellular state.<\/jats:p>","DOI":"10.1002\/bit.28053","type":"journal-article","created":{"date-parts":[[2022,2,4]],"date-time":"2022-02-04T12:25:21Z","timestamp":1643977521000},"page":"1222-1238","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Extracellular vesicles facilitate large\u2010scale dynamic exchange of proteins and RNA among cultured Chinese hamster ovary and human cells"],"prefix":"10.1002","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3466-6428","authenticated-orcid":false,"given":"Jessica","family":"Belliveau","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering University of Delaware Newark Delaware USA"},{"name":"Delaware Biotechnology Institute University of Delaware Newark Delaware 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