{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T14:57:12Z","timestamp":1763564232270,"version":"build-2065373602"},"reference-count":43,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T00:00:00Z","timestamp":1659657600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>With the increasingly serious drug resistance of <jats:italic>Acinetobacter baumannii<\/jats:italic>, there is an increasingly urgent need for new antibacterial drugs. Phage lysin PlyAB1 has a bactericidal effect on drug\u2010resistant <jats:italic>A. baumannii<\/jats:italic>, which has the potential to replace antibiotics to fight infection caused by <jats:italic>A. baumannii<\/jats:italic>. However, its application is limited by its thermal stability and lytic activity. To solve these problems, molecular dynamics (MD) simulations combined with Hotspot wizard 3.0 were used to identify key residue sites affecting thermal stability, and evolutionary analysis combined with multiple sequence alignment was used to identify key residue sites affecting lytic activity. Four single\u2010point variants with significantly increased thermal stability and four single\u2010point variants with significantly lytic activity were obtained, respectively. Furthermore, by superimposing mutations, we obtained three double\u2010point variants, G100Q\/K69R, G100R\/K69R, and G100K\/K69R, with significantly improved thermal stability and improved lytic activity. At 45\u00b0C,\u00a0the lytic activity and half\u2010life of the optimal variant G100Q\/K69R were 1.51\u2010\u00a0and 24\u2010fold\u00a0higher than those of the wild PlyAB1, respectively. These results deepen our understanding of the structure and function of phage lysin and contribute to the application of phage lysin in antibiotic substitution.<\/jats:p>","DOI":"10.1002\/bit.28187","type":"journal-article","created":{"date-parts":[[2022,7,20]],"date-time":"2022-07-20T22:18:47Z","timestamp":1658355527000},"page":"2731-2742","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Enhancing thermal stability and lytic activity of phage lysin PlyAB1 from <i>Acinetobacter baumannii<\/i>"],"prefix":"10.1002","volume":"119","author":[{"given":"Yingbo","family":"Yuan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Technology Shandong University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2737-012X","authenticated-orcid":false,"given":"Qingbin","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology Shandong University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuhang","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology Shandong University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinhong","family":"Gu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology Shandong University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangtao","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Burn and Plastic Surgery, Shenzhen Institute of Translational Medicine, The First Affiliated Hospital of Shenzhen University Shenzhen Second People's Hospital Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9015-4561","authenticated-orcid":false,"given":"Qingsheng","family":"Qi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology Shandong University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuemei","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology Shandong University Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw416"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.25637"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg500"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0079800"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471250953.bi0506s15"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1042\/Bst20150192"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00007"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.148"},{"key":"e_1_2_8_10_1","doi-asserted-by":"crossref","unstructured":"Heselpoth R. 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