{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T07:15:03Z","timestamp":1763968503652,"version":"build-2065373602"},"reference-count":17,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,1,12]],"date-time":"2023-01-12T00:00:00Z","timestamp":1673481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Reducing drug development timelines is an industry\u2010wide goal to bring medicines to patients in need more quickly. This was exemplified in the coronavirus disease 2019 pandemic where reducing development timelines had a direct impact on the number of lives lost to the disease. The use of drug substances produced using cell pools, as opposed to clones, has the potential to shorten development timelines. Toward this goal, we have developed a novel technology, GPEx\u00ae Lightning, that allows for rapid, reproducible, targeted recombination of transgenes into more than 200 Dock sites in the Chinese hamster ovary cell line\u00a0genome. This allows for rapid production of high\u2010expressing stable cell pools and clones that reach titers of 4\u201312\u2009g\/l in generic fed\u2010batch production. These pools and clones are highly stable in both titer and glycosylation, showing strong similarities in glycosylation profiles.<\/jats:p>","DOI":"10.1002\/bit.28323","type":"journal-article","created":{"date-parts":[[2022,12,29]],"date-time":"2022-12-29T23:22:53Z","timestamp":1672356173000},"page":"2297-2307","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Shortening the biologics clinical timeline with a novel method for generating stable, high\u2010producing cell pools and clones"],"prefix":"10.1002","volume":"122","author":[{"given":"Chad A.","family":"Hall","sequence":"first","affiliation":[{"name":"Catalent Pharma Solutions Somerset New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel H.","family":"Kravitz","sequence":"additional","affiliation":[{"name":"Catalent Pharma Solutions Somerset New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl F.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Catalent Pharma Solutions Somerset New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas A.","family":"Sanek","sequence":"additional","affiliation":[{"name":"Catalent Pharma Solutions Somerset New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Payel","family":"Maiti","sequence":"additional","affiliation":[{"name":"Catalent Pharma Solutions Somerset New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith R.","family":"Ziemba","sequence":"additional","affiliation":[{"name":"Catalent Pharma Solutions Somerset New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Liu","sequence":"additional","affiliation":[{"name":"Catalent Pharma Solutions Somerset New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitri O.","family":"Andreev","sequence":"additional","affiliation":[{"name":"Catalent Pharma Solutions Somerset New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria L.","family":"Chrostowski","sequence":"additional","affiliation":[{"name":"Catalent Pharma Solutions Somerset New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian J.","family":"Collins","sequence":"additional","affiliation":[{"name":"Catalent Pharma Solutions Somerset New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory T.","family":"Bleck","sequence":"additional","affiliation":[{"name":"Catalent Pharma Solutions Somerset New Jersey USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,1,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.27995"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.12665\/j54.bleck"},{"issue":"5","key":"e_1_2_9_4_1","first-page":"56","article-title":"Consistent production of genetically stable mammalian cell lines","volume":"25","author":"Bleck G.","year":"2012","journal-title":"BioPharm International"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2009.12.007"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.3137"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr436"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/gen.39.07.16"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0020234"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.3140"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.2495"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.27742"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2022.03.010"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coche.2018.09.010"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-922-2:099"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1083413"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2021.2005507"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.3186"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.28323","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bit.28323","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.28323","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:34:12Z","timestamp":1760038452000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.28323"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,12]]},"references-count":17,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1002\/bit.28323"],"URL":"https:\/\/doi.org\/10.1002\/bit.28323","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"type":"print","value":"0006-3592"},{"type":"electronic","value":"1097-0290"}],"subject":[],"published":{"date-parts":[[2023,1,12]]},"assertion":[{"value":"2022-12-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}