{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:51:32Z","timestamp":1792511492154,"version":"4.5.0"},"reference-count":51,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T00:00:00Z","timestamp":1681430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006087","name":"Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico, Universidad Nacional Aut\u00f3noma de M\u00e9xico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006087","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vaccine development against dengue virus is challenging because of the antibody\u2010dependent enhancement of infection (ADE), which causes severe disease. Consecutive infections by Zika (ZIKV) and\/or dengue viruses (DENV), or vaccination can predispose to ADE. Current vaccines and vaccine candidates contain the complete envelope viral protein, with epitopes that can raise antibodies causing ADE. We used the envelope dimer epitope (EDE), which induces neutralizing antibodies that do not elicit ADE, to design a vaccine against both flaviviruses. However, EDE is a discontinuous quaternary epitope that cannot be isolated from the E protein without other epitopes. Utilizing phage display, we selected three peptides that mimic the EDE. Free mimotopes were disordered and did not elicit an immune response. After their display on adeno\u2010associated virus (AAV) capsids (VLP), they recovered their structure and were recognized by an EDE\u2010specific antibody. Characterization by cryo\u2010EM and enzyme\u2010linked immunosorbent assay confirmed the correct display of a mimotope on the surface of the AAV VLP and its recognition by the specific antibody. Immunization with the AAV VLP displaying one of the mimotopes induced antibodies that recognized ZIKV and DENV. This work provides the basis for developing a Zika and dengue virus vaccine candidate that will not induce ADE.<\/jats:p>","DOI":"10.1002\/bit.28392","type":"journal-article","created":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T12:39:06Z","timestamp":1681475946000},"page":"2658-2671","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Mimotope discovery as a tool to design a vaccine against Zika and dengue viruses"],"prefix":"10.1002","volume":"120","author":[{"given":"Esmeralda","family":"Cuevas\u2010Ju\u00e1rez","sequence":"first","affiliation":[{"name":"Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Morelos Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arturo","family":"Li\u00f1an\u2010Torres","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Morelos Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"National Center for In\u2010situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center New York Structural Biology Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mykhailo","family":"Kopylov","sequence":"additional","affiliation":[{"name":"National Center for In\u2010situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center New York Structural Biology Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clint S.","family":"Potter","sequence":"additional","affiliation":[{"name":"National Center for In\u2010situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center New York Structural Biology Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bridget","family":"Carragher","sequence":"additional","affiliation":[{"name":"National Center for In\u2010situ Tomographic Ultramicroscopy, Simons Electron Microscopy Center New York Structural Biology Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Octavio T.","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Morelos Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura A.","family":"Palomares","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico  Morelos 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