{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T03:14:10Z","timestamp":1780542850783,"version":"3.54.1"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T00:00:00Z","timestamp":1695600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The lactic acid bacterium <jats:italic>Limosilactobacillus reuteri<\/jats:italic> (formerly <jats:italic>Lactobacillus reuteri<\/jats:italic>) is a desirable host for the production of 1,3\u2010propanediol (1,3\u2010PDO) from glycerol when 1,3\u2010PDO is used in the food or cosmetic industry. However, the production is hindered by strain instability, causing cell lysis, and difficult gene manipulation. This study reveals that the stability of <jats:italic>L. reuteri<\/jats:italic> DSM 20016 and its 1,3\u2010PDO production, especially in the alcohol dehydrogenases (ADHs)\u2010deletion mutants, are greatly enhanced after the deletion of two prophages (\u03a63 and \u03a64) present in the <jats:italic>L. reuteri<\/jats:italic>'s chromosome. The resulting phage\u2010free and ADHs\u2010deletion mutant could produce &gt;825\u2009mM 1,3\u2010PDO in 48\u2009h without cell lysis at the theoretical maximum yield on glucose of ~2\u2009mol\/mol. Compared to the wild\u2010type strain, the mutant exhibited a 45.2% increase in 1,3\u2010PDO production titer and a 2.1\u2010fold increase in yield. In addition, this study reports that the transformation efficiency of <jats:italic>L. reuteri<\/jats:italic> \u0394<jats:italic>adh2<\/jats:italic>\u0394<jats:italic>adh6<\/jats:italic> mutant strains were greatly enhanced by &gt;300\u2010fold after the deletion of prophage \u03a63, probably due to the removal of a restriction\u2010modification (RM) system which resides in the phage genome. With improved stability and higher transformation efficiency, recombinant <jats:italic>L. reuteri<\/jats:italic> DSM 20016 \u0394<jats:italic>adh<\/jats:italic>2\u0394<jats:italic>adh<\/jats:italic>6\u0394\u03a63\u0394\u03a64 can be a more reliable and amenable host for industrial applications.<\/jats:p>","DOI":"10.1002\/bit.28559","type":"journal-article","created":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T11:06:27Z","timestamp":1695639987000},"page":"317-328","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Construction of prophage\u2010free and highly\u2010transformable <i>Limosilactobacillus reuteri<\/i> strains and their use for production of 1,3\u2010propanediol"],"prefix":"10.1002","volume":"121","author":[{"given":"Kalpana","family":"Singh","sequence":"first","affiliation":[{"name":"School of Energy and Chemical Engineering, UNIST Ulsan Republic of 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