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Here, we report the biostimulated (BIOST: addition of lactate) and natural attenuated (NAT) degradation of chloroethene compounds under microoxic conditions by bacterial communities from chloroethene compounds\u2010contaminated groundwater. The degradation of tetrachloroethene was significantly higher in NAT (15.14% on average) than in BIOST (10.13% on average) conditions at the end of the experiment (90 days). <jats:italic>Sporomusa<\/jats:italic>, <jats:italic>Paracoccus<\/jats:italic>, <jats:italic>Sedimentibacter<\/jats:italic>, <jats:italic>Pseudomonas<\/jats:italic>, and <jats:italic>Desulfosporosinus<\/jats:italic> were overrepresented in NAT and BIOST compared to the source groundwater. The NAT metagenome contains phenol hydrolase P1 oxygenase (<jats:italic>dmpL<\/jats:italic>), catechol\u20101,2\u2010dioxygenase (<jats:italic>catA<\/jats:italic>), catechol\u20102,3\u2010dioxygenases (<jats:italic>dmpB, todE<\/jats:italic>, and <jats:italic>xylE<\/jats:italic>) genes, which could be involved in the cometabolic degradation of chloroethene compounds; and chlorate reductase (<jats:italic>clrA<\/jats:italic>), that could be associated with partial reductive dechlorination of chloroethene compounds. Our data provide a better understanding of the bacterial communities, genes, and pathways potentially implicated in the reductive and cometabolic degradation of chloroethene compounds under microoxic conditions.<\/jats:p>","DOI":"10.1002\/bit.28630","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T05:24:28Z","timestamp":1703049868000},"page":"1036-1049","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Biodegradation of chloroethene compounds under microoxic conditions"],"prefix":"10.1002","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2851-8082","authenticated-orcid":false,"given":"Abidemi Oluranti","family":"Ojo","sequence":"first","affiliation":[{"name":"Department of Microbial, Biochemical and Food Biotechnology University of the Free State Bloemfontein South Africa"},{"name":"Centre for Applied Food Sustainability and Biotechnology Central University of Technology Bloemfontein South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio","family":"Castillo","sequence":"additional","affiliation":[{"name":"Department of Microbial, Biochemical and Food Biotechnology University of the Free State Bloemfontein South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1598-6151","authenticated-orcid":false,"given":"Errol Duncan","family":"Cason","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences University of the Free State Bloemfontein South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angel","family":"Valverde","sequence":"additional","affiliation":[{"name":"Instituto de Recursos Naturales y Agrobiolog\u00eda de Salamanca (IRNASA\u2010CSIC) Salamanca Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,12,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/es801759k"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gca.2020.01.055"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-75"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/10889860390246141"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth0510-335"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-017-2243-4"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.4172\/2329-6674.1000e102"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2004.05.004"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/es702422d"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/es903535j"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep30766"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2021.106401"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12413"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-7722(01)00217-0"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-017-8867-y"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq461"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.9.3038-3046.1992"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/mr.58.4.641-685.1994"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00215-07"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.60218"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2019.115431"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2018.12.022"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012431313025"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconhyd.2013.04.002"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.41"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-023-29839-1"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2020.126842"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050577"},{"key":"e_1_2_9_31_1","unstructured":"IARC. 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