{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:25:33Z","timestamp":1790270733028,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T00:00:00Z","timestamp":1712534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Microbial production of polyhydroxyalkanoate (PHA) is greatly restricted by high production cost arising from high\u2010temperature sterilization and expensive carbon sources. In this study, a low\u2010cost PHA production platform was established from\n                    <jats:italic>Halomonas cupida<\/jats:italic>\n                    J9. First, a marker\u2010less genome\u2010editing system was developed in\n                    <jats:italic>H. cupida<\/jats:italic>\n                    J9. Subsequently,\n                    <jats:italic>H. cupida<\/jats:italic>\n                    J9 was engineered to efficiently utilize xylose for PHA biosynthesis by introducing a new xylose metabolism module and blocking xylonate production. The engineered strain J9U\u0394\n                    <jats:italic>xylD<\/jats:italic>\n                    \u2010P8\n                    <jats:italic>xylA<\/jats:italic>\n                    has the highest PHA yield (2.81\u2009g\/L) obtained by\n                    <jats:italic>Halomonas<\/jats:italic>\n                    with xylose as the sole carbon source so far. This is the first report on the production of short\u2010 and medium\u2010chain\u2010length (SCL\u2010\n                    <jats:italic>co<\/jats:italic>\n                    \u2010MCL) PHA from xylose by\n                    <jats:italic>Halomonas<\/jats:italic>\n                    . Interestingly, J9U\u0394\n                    <jats:italic>xylD<\/jats:italic>\n                    \u2010P8\n                    <jats:italic>xylA<\/jats:italic>\n                    was capable of efficiently utilizing glucose and xylose as co\u2010carbon sources for PHA production. Furthermore, fed\u2010batch fermentation of J9U\u0394\n                    <jats:italic>xylD<\/jats:italic>\n                    \u2010P8\n                    <jats:italic>xylA<\/jats:italic>\n                    coupled to a glucose\/xylose co\u2010feeding strategy reached up to 12.57\u2009g\/L PHA in a 5\u2010L bioreactor under open and unsterile condition. Utilization of corn straw hydrolysate as the carbon source by J9U\u0394\n                    <jats:italic>xylD<\/jats:italic>\n                    \u2010P8\n                    <jats:italic>xylA<\/jats:italic>\n                    reached 7.0\u2009g\/L cell dry weight (CDW) and 2.45\u2009g\/L PHA in an open fermentation. In summary, unsterile production in combination with inexpensive feedstock highlights the potential of the engineered strain for the low\u2010cost production of PHA from lignocellulose\u2010rich agriculture waste.\n                  <\/jats:p>","DOI":"10.1002\/bit.28694","type":"journal-article","created":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T06:29:58Z","timestamp":1712557798000},"page":"2106-2120","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Establishment of low\u2010cost production platforms of polyhydroxyalkanoate bioplastics from\n                    <i>Halomonas cupida<\/i>\n                    J9"],"prefix":"10.1002","volume":"121","author":[{"given":"Siqi","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongfu","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Meng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weini","family":"Xiong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruihua","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, College of Life Sciences Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7607-6621","authenticated-orcid":false,"given":"Chao","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences Nankai University Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2021.04.171"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2022.159333"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2021.126195"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx343"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-022-12215-3"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2018.05.019"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2020.08.001"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03140.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-14024-1"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02502-08"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclepro.2020.122521"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.05055-11"},{"key":"e_1_2_8_14_1","volume-title":"Molecular cloning: A laboratory manual","author":"Green M. 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