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The key distinction from other microbial bioprocesses lies in the critical role that light plays in cultures, as it serves as a source of environmental information to control metabolic processes. Therefore, we can use these criteria to design a bioprocess that aims to stimulate the accumulation of target molecules by controlling light exposure. We study the effect on biochemical and photobiological responses of <jats:italic>Golenkinia brevispicula<\/jats:italic> FAUBA\u20103 to the exposition of different spectral irradiances (specifically, high\u2010fluence PAR of narrow yellow spectrum complemented with low intensity of monochromatic radiations of red, blue, and UV\u2010A) under prestress and salinity stress conditions. High light (HL) intensity coupled to salinity stress affected the photosynthetic activity and photoprotection mechanisms as shown by maximal quantum yield (<jats:italic>F<\/jats:italic><jats:sub><jats:italic>v<\/jats:italic><\/jats:sub><jats:italic>\/F<\/jats:italic><jats:sub><jats:italic>m<\/jats:italic><\/jats:sub>) and non\u2010photochemical quenching (NPQ<jats:sub>max<\/jats:sub>) reduction, respectively. HL treatments combined with the proper dose of UV\u2010A radiation under salinity stress induced the highest carotenoid content (2.75\u2009mg\u2009g dry weight [DW]<jats:sup>\u2212<\/jats:sup><jats:sup>1<\/jats:sup>) composed mainly of lutein and \u03b2\u2010carotene, and the highest lipid accumulation (35.3% DW) with the highest polyunsaturated fatty acid content (alpha\u2010linolenic acid (C18:3) and linoleic acid (C18:2)). Our study can guide the strategies for commercial indoor production of <jats:italic>G. brevispicula<\/jats:italic> for high\u2010value metabolites.<\/jats:p>","DOI":"10.1002\/bit.28831","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T01:58:49Z","timestamp":1724637529000},"page":"3715-3727","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Increase in polyunsaturated fatty acids and carotenoid accumulation in the microalga <i>Golenkinia brevispicula<\/i> (Chlorophyceae) by manipulating spectral irradiance and salinity"],"prefix":"10.1002","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0690-7191","authenticated-orcid":false,"given":"T. A.","family":"Rearte","sequence":"first","affiliation":[{"name":"C\u00e1tedra de Qu\u00edmica Inorg\u00e1nica y Anal\u00edtica, Departamento de Recursos Naturales y Ambiente, Facultad de Agronom\u00eda Universidad de Buenos Aires, CABA Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. S. M.","family":"Celis\u2010Pla","sequence":"additional","affiliation":[{"name":"Laboratory of Aquatic Environmental Research (LACER)\/HUB\u2010AMBIENTAL UPLA Playa Ancha University Valpara\u00edso Chile"},{"name":"Departamento de Ciencias y Geograf\u00eda, Facultad de Ciencias Naturales y Exactas Universidad de Playa Ancha Valpara\u00edso Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Abdala\u2010D\u00edaz","sequence":"additional","affiliation":[{"name":"Universidad de M\u00e1laga, Instituto Andaluz de Biotecnolog\u00eda y Desarrollo Azul (IBYDA), Centro Experimental Grice Hutchinson M\u00e1laga Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Castro\u2010Varela","sequence":"additional","affiliation":[{"name":"Departamento de Bot\u00e1nica, Facultad de Ciencias Naturales y Oceanogr\u00e1ficas Universidad de Concepci\u00f3n Concepci\u00f3n Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. N.","family":"Marsili","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Qu\u00edmica Inorg\u00e1nica y Anal\u00edtica, Departamento de Recursos Naturales y Ambiente, Facultad de Agronom\u00eda Universidad de Buenos Aires, CABA Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas (CONICET) Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Universidad Nacional de Cuyo Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8538-145X","authenticated-orcid":false,"given":"M. C.","family":"Cer\u00f3n\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Research Centre CIAIMBITAL University of Almer\u00eda Almer\u00eda Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. 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