{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T23:53:08Z","timestamp":1788479588364,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Enantiopure 1,2\u2010diols are widely used in the production of pharmaceuticals, cosmetics, and functional materials as essential building blocks or bioactive compounds. Nevertheless, developing a mild, efficient and environmentally friendly biocatalytic route for manufacturing enantiopure 1,2\u2010diols from simple substrate remains a challenge. Here, we designed and realized a step\u2010wise biocatalytic cascade to access chiral 1,2\u2010diols starting from aromatic aldehyde and formaldehyde enabled by a newly mined benzaldehyde lyase from <jats:italic>Sphingobium sp<\/jats:italic>. combined with a pair of tailored\u2010made short\u2010chain dehydrogenase\/reductase from <jats:italic>Pseudomonas monteilii<\/jats:italic> (PmSDR\u2010MuR and PmSDR\u2010MuS) capable of producing (<jats:italic>R<\/jats:italic>)\u2010 and (<jats:italic>S<\/jats:italic>)\u20101\u2010phenylethane\u20101,2\u2010diol with 99% <jats:italic>ee<\/jats:italic>. The planned biocatalytic cascade could synthesize a series of enantiopure 1,2\u2010diols with a broad scope (16 samples), excellent conversions (94%\u201399%), and outstanding enantioselectivity (up to 99% <jats:italic>ee<\/jats:italic>), making it an effective technique for producing chiral 1,2\u2010diols in a more environmentally friendly and sustainable manner.<\/jats:p>","DOI":"10.1002\/bit.28841","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T06:21:04Z","timestamp":1725949264000},"page":"3796-3807","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Rational design of short\u2010chain dehydrogenase\/reductase for enantio\u2010complementary synthesis of chiral 1,2\u2010diols by successive hydroxymethylation and reduction of aldehydes"],"prefix":"10.1002","volume":"121","author":[{"given":"Xiu\u2010Xin","family":"Ren","sequence":"first","affiliation":[{"name":"College of 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