{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T10:27:49Z","timestamp":1788776869839,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T00:00:00Z","timestamp":1730332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>1,5\u2010Pentanediol (1,5\u2010PDO) is an important five\u2010carbon alcohol, widely used in polymer and pharmaceutical industries. Considering the substantial energy (ATP and NADPH) requirements of previous pathways, an energy\u2010conserving artificial pathway with a higher theoretical yield (0.75\u2009mol\/mol glucose) was designed and constructed in this study. In this pathway, lysine is converted into 1,5\u2010PDO by decarboxylation, two transamination, and two reduction reactions. For the purpose of full pathway construction, 5\u2010aminopetanal reductase and 5\u2010amino\u20101\u2010pentanol (5\u2010APO) transaminase were identified and characterized. By implementing strategies such as modular optimization of gene expression, enhancing lysine biosynthesis and increasing NADPH supply, the engineered strains were able to produce 1502.8\u2009mg\/L 5\u2010APO and 726.2\u2009mg\/L 1,5\u2010PDO in shake flasks and 11.7\u2009g\/L 1,5\u2010PDO in a 3\u2009L bioreactor. This work provides a new and promising pathway for the efficient production of 5\u2010APO and 1,5\u2010PDO.<\/jats:p>","DOI":"10.1002\/bit.28875","type":"journal-article","created":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:29:25Z","timestamp":1730420965000},"page":"445-451","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Design an Energy\u2010Conserving Pathway for Efficient Biosynthesis of 1,5\u2010Pentanediol and 5\u2010Amino\u20101\u2010Pentanol"],"prefix":"10.1002","volume":"122","author":[{"given":"Lin","family":"Ma","sequence":"first","affiliation":[{"name":"State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical Technology Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Resource Engineering Beijing University of 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