{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T02:54:05Z","timestamp":1783824845779,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T00:00:00Z","timestamp":1733788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Measurement of antibody and antibody fusion protein concentration is vital for process development and manufacturing. Continuous, in\u2010line monitoring of antibody concentration could be useful in a variety of applications, such as controlling the loading of protein A columns to prevent breakthrough, monitoring bioreactor titer, and detecting leaks past ultrafiltration\/diafiltration membranes. Molecule\u2010specific monitoring techniques are advantageous for antibody detection in cell culture fluid in the presence of complex process impurities. Here we report a continuous in\u2010line, real\u2010time IgG monitoring platform using a fiber\u2010optic biosensor with a replaceable sensor tip covalently functionalized with a fluor\u2010labeled protein consisting of a pentamer of the Z domain (a more stable form of the B domain) of protein A. The sensor demonstrates concentration\u2010dependent fluorescence enhancement in the presence of human IgG (0.01\u20130.75\u2009g\/L), with consistent signals during five runs each with 1 and 0.1\u2009g\/L IgG, and maintains its specificity in the presence of Chinese hamster ovary (CHO) cell culture fluid. A 5% breakthrough of a typical 10\u2009g\/L load would be detected in less than 20\u2009s in a flowing stream emerging from a protein A column without prior sample preparation. This sensor platform may be suitable for monitoring IgG and fragment, crystallizable (Fc) fusion proteins in diverse upstream and downstream bioprocess applications.<\/jats:p>","DOI":"10.1002\/bit.28900","type":"journal-article","created":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T00:48:13Z","timestamp":1733878093000},"page":"538-547","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Fluorescent Reporter\u2010Based Fiber Optic Probe for Continuous Detection of Antibodies"],"prefix":"10.1002","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7963-6209","authenticated-orcid":false,"given":"Suman","family":"Nandy","sequence":"first","affiliation":[{"name":"William A. Brookshire Department of Chemical and Biomolecular Engineering University of Houston Houston Texas USA"},{"name":"Biologics Process R&amp;D Merck &amp; Co., Inc. Rahway NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5054-2890","authenticated-orcid":false,"given":"Binh V.","family":"Vu","sequence":"additional","affiliation":[{"name":"William A. Brookshire Department of Chemical and Biomolecular Engineering University of Houston Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6565-9277","authenticated-orcid":false,"given":"Vijay M.","family":"Maranholkar","sequence":"additional","affiliation":[{"name":"Department of Biology and Biochemistry University of Houston Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7385-7692","authenticated-orcid":false,"given":"Atul","family":"Goyal","sequence":"additional","affiliation":[{"name":"William A. Brookshire Department of Chemical and Biomolecular Engineering University of Houston Houston Texas USA"},{"name":"Vaccine Process R&amp;D Pfizer Pearl River NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5891-6415","authenticated-orcid":false,"given":"Katerina","family":"Kourentzi","sequence":"additional","affiliation":[{"name":"William A. Brookshire Department of Chemical and Biomolecular Engineering University of Houston Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0900-6148","authenticated-orcid":false,"given":"Richard C.","family":"Willson","sequence":"additional","affiliation":[{"name":"William A. Brookshire Department of Chemical and Biomolecular Engineering University of Houston Houston Texas USA"},{"name":"Department of Biology and Biochemistry University of Houston Houston Texas USA"},{"name":"Escuela de Medicina y Ciencias de Salud, Tecnol\u00f3gico de Monterrey Monterrey Nuevo Le\u00f3n Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.14891\/ANALSCISP.17ICAS.0.I1469.0"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.2c00311"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/S100470200010\/METRICS"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep30526"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/PNAS.93.12.5688"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CES.2017.10.030"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/BTPR.2250"},{"issue":"077","key":"e_1_2_10_9_1","first-page":"210","article-title":"Immobilization of Active Agents on Substrates With a Silane and Heterobifunctional Crosslinking Agent","volume":"5","author":"Eigler F. S.","year":"1991","journal-title":"U.S. Patent No."},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/BTPR.2847"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b04561"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/BIT.28254"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CHROMA.2010.06.007"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389203722666211210121258"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja017074a"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.AB.2020.113899"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/BIOS11100383"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2313(75)90003-4"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm050601o"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/01496395.2013.767837"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/S91008271"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2020.112327"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/19420862.2021.1980178"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.0c00791"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0687-604"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/BIT.27894"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/BIT.26078"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2021.111916"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201701481"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.BIOS.2011.02.007"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2004.10.026"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/BIT.27236"},{"key":"e_1_2_10_33_1","unstructured":"Thermo Scientific 2011. Calculate dye:protein (F\/P) molar ratios [WWW Document]. Thermo Scientific TECH TIP #31 0747. URLhttps:\/\/tools.thermofisher.com\/content\/sfs\/brochures\/TR0031-Calc-FP-ratios.pdf(accessed May 25 2024)."},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/PR8101302"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2AY01567F"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c03446"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzx029"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.22541\/au.167311811.10102371\/v1"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/membranes13100815"}],"container-title":["Biotechnology and Bioengineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bit.28900","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T22:56:52Z","timestamp":1760050612000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bit.28900"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,10]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1002\/bit.28900"],"URL":"https:\/\/doi.org\/10.1002\/bit.28900","archive":["Portico"],"relation":{},"ISSN":["0006-3592","1097-0290"],"issn-type":[{"value":"0006-3592","type":"print"},{"value":"1097-0290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,10]]},"assertion":[{"value":"2024-08-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}