{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T15:25:01Z","timestamp":1791213901414,"version":"4.1.0"},"reference-count":63,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T00:00:00Z","timestamp":1735257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T00:00:00Z","timestamp":1735257600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech &amp; Bioengineering"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Acetogenic bacteria play an important role in various biotechnological processes, because of their chemolithoautotrophic metabolism converting carbon dioxide with molecular hydrogen (H\n                    <jats:sub>2<\/jats:sub>\n                    ) as electron donor into acetate. As the main factor limiting acetogenesis is often H\n                    <jats:sub>2<\/jats:sub>\n                    , insights into the H\n                    <jats:sub>2<\/jats:sub>\n                    consumption kinetics of acetogens are required to assess their potential in biotechnological processes. In this study, initial H\n                    <jats:sub>2<\/jats:sub>\n                    consumption rates at a range of different initial H\n                    <jats:sub>2<\/jats:sub>\n                    concentrations were measured for three different acetogens. Interestingly, for all three strains, H\n                    <jats:sub>2<\/jats:sub>\n                    consumption was found to follow first\u2010order kinetics, i.e. the H\n                    <jats:sub>2<\/jats:sub>\n                    consumption rate increased linearly with the dissolved H\n                    <jats:sub>2<\/jats:sub>\n                    concentration, up to almost saturated H\n                    <jats:sub>2<\/jats:sub>\n                    levels (600\u2009\u00b5M). This is in contrast with Monod kinetics and low half\u2010saturation concentrations, which have commonly been assumed for acetogens. The obtained biomass specific first\u2010order rate coefficients (\n                    <jats:italic>k<\/jats:italic>\n                    <jats:sub>1<\/jats:sub>\n                    <jats:sup>X<\/jats:sup>\n                    ) were further validated by comparison with values obtained by fitting first\u2010order kinetics on previous time\u2010course experimental results. The latter method was also used to determine the\n                    <jats:italic>k<\/jats:italic>\n                    <jats:sub>1<\/jats:sub>\n                    <jats:sup>X<\/jats:sup>\n                    value of five additional acetogens strains. Biomass specific first\u2010order rate coefficients were found to vary up to six\u2010fold, with the highest\n                    <jats:italic>k<\/jats:italic>\n                    <jats:sub>1<\/jats:sub>\n                    <jats:sup>X<\/jats:sup>\n                    for\n                    <jats:italic>Acetobacterium wieringae<\/jats:italic>\n                    and the lowest for\n                    <jats:italic>Sporomusa sphaeroides<\/jats:italic>\n                    . Overall, our results demonstrate the importance of the dissolved H\n                    <jats:sub>2<\/jats:sub>\n                    concentration to understand the rate of acetogenesis in biotechnological systems.\n                  <\/jats:p>","DOI":"10.1002\/bit.28904","type":"journal-article","created":{"date-parts":[[2024,12,28]],"date-time":"2024-12-28T01:28:41Z","timestamp":1735349321000},"page":"804-816","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["H\n                    <sub>2<\/sub>\n                    Consumption by Various Acetogenic Bacteria Follows First\u2010Order Kinetics up to H\n                    <sub>2<\/sub>\n                    Saturation"],"prefix":"10.1002","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0705-9999","authenticated-orcid":false,"given":"Laura","family":"Mu\u00f1oz\u2010Duarte","sequence":"first","affiliation":[{"name":"Department of Biological and Chemical Engineering Aarhus University Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susmit","family":"Chakraborty","sequence":"additional","affiliation":[{"name":"Department of Biological and Chemical Engineering Aarhus University Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise Vinther","family":"Gr\u00f8n","sequence":"additional","affiliation":[{"name":"Department of Biological and Chemical Engineering Aarhus University Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Florencia","family":"Bambace","sequence":"additional","affiliation":[{"name":"Department of Biological and Chemical Engineering Aarhus University Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4819-5436","authenticated-orcid":false,"given":"Jacopo","family":"Catalano","sequence":"additional","affiliation":[{"name":"Department of Biological and Chemical Engineering Aarhus University Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0471-7802","authenticated-orcid":false,"given":"Jo","family":"Philips","sequence":"additional","affiliation":[{"name":"Department of Biological and Chemical Engineering Aarhus University Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00303591"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2022.07.004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00058"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3303\/CET1864026"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1186930"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/BS.AAMBS.2018.01.002"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/d3gc00471f"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuab008"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00407793"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41929\u2010019\u20100272\u20100"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511805134"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.8.4468\u20104473.1990"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050285"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201037630\u2010y"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020514617738"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012373944-5.00260-1"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.53.3.471\u2010476.1987"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2016.04.032"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00449\u2010016\u20101634\u20105"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2024.02.004"},{"key":"e_1_2_8_22_1","volume-title":"CRC Handbook of Chemistry and Physics","author":"Haynes W. 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